Publication detail

Rapid and Ultrasensitive Method for Determination of Phytochelatin2 using High Performance Liquid Chromatography with Electrochemical Detection

ZÍTKA, O. ŠKUTKOVÁ, H. KRYŠTOFOVÁ, O. ŠOBROVÁ, P. ADAM, V. ZEHNÁLEK, J. BEKLOVÁ, M. HUBÁLEK, J. PROVAZNÍK, I. KIZEK, R.

Original Title

Rapid and Ultrasensitive Method for Determination of Phytochelatin2 using High Performance Liquid Chromatography with Electrochemical Detection

English Title

Rapid and Ultrasensitive Method for Determination of Phytochelatin2 using High Performance Liquid Chromatography with Electrochemical Detection

Type

journal article in Web of Science

Language

en

Original Abstract

Plants respond to heavy metal toxicity in a variety of different ways including synthesis of phytochelatins. The synthesis of phytochelatins is catalyzed by gama-Glu-Cys dipeptidyl transpeptidase named as phytochelatin synthase (PCS). The main aim of this study was to optimize high performance liquid chromatography coupled with electrochemical detector for determination of phytochelatin2. The optimized procedure was subsequently used for determination of the mentioned molecules with special attention aimed at the possibility to determine PC2 after activation of PCS in the tobacco BY-2 cells treated with different concentrations of cadmium(II) ions.

English abstract

Plants respond to heavy metal toxicity in a variety of different ways including synthesis of phytochelatins. The synthesis of phytochelatins is catalyzed by gama-Glu-Cys dipeptidyl transpeptidase named as phytochelatin synthase (PCS). The main aim of this study was to optimize high performance liquid chromatography coupled with electrochemical detector for determination of phytochelatin2. The optimized procedure was subsequently used for determination of the mentioned molecules with special attention aimed at the possibility to determine PC2 after activation of PCS in the tobacco BY-2 cells treated with different concentrations of cadmium(II) ions.

Keywords

cadmium, glutathione, phytochelatin, high performance liquid chromatography, electrochemical detector, tobacco BY-2 cell suspension

RIV year

2011

Released

01.05.2011

Pages from

1367

Pages to

1381

Pages count

15

BibTex


@article{BUT50164,
  author="Ondřej {Zítka} and Helena {Škutková} and Olga {Kryštofová} and Pavlína {Adam} and Vojtěch {Adam} and Josef {Zehnálek} and Miroslava {Beklová} and Jaromír {Hubálek} and Ivo {Provazník} and René {Kizek}",
  title="Rapid and Ultrasensitive Method for Determination of Phytochelatin2 using High Performance Liquid Chromatography with Electrochemical Detection",
  annote="Plants respond to heavy metal toxicity in a variety of different ways including synthesis of phytochelatins. The synthesis of phytochelatins is catalyzed by gama-Glu-Cys dipeptidyl transpeptidase named as phytochelatin synthase (PCS). The main aim of this study was to optimize high performance 
liquid chromatography coupled with electrochemical detector for determination of phytochelatin2. The optimized procedure was subsequently used for determination of the mentioned molecules with special attention aimed at the possibility to determine PC2 after activation of PCS in the tobacco BY-2 cells treated with different concentrations of cadmium(II) ions.",
  chapter="50164",
  number="5",
  volume="2011 (6)",
  year="2011",
  month="may",
  pages="1367--1381",
  type="journal article in Web of Science"
}