Publication detail

Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions

Vaclav Diopan, Violetta Shestivska, Ondrej Zitka, Michaela Galiova, Vojtech Adam, Jozef Kaiser, Ales Horna, Karel Novotny, Miroslav Liska, Ladislav Havel, Josef Zehnalek, Rene Kizek

Original Title

Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions

Type

journal article - other

Language

English

Original Abstract

The main aim of this paper is to utilize high performance liquid chromatography with electrochemical detection for determination of thiols content in plants tissues of lettuce treated with lead(II) ions (0, 0.5 and 1 mM). We used two HPLC-ED instruments: HPLC coupled with one channel amperometric detector and HPLC coupled with twelve channel coulometric detector to detect simultaneously twelve thiols. The detection limits of thiols measured by CoulArray detector were about two magnitudes lower in comparison to those measured by Coulochem III detector and were from tens to hundreds pM. Under the optimal conditions, we utilized HPLC-CoulArray detector for analysis of tissues from lettuce plants. In addition, distribution and accumulation of lead ions with high spatial resolution was monitored using laser induced breakdown spectroscopy.

Keywords

Thiols, Liquid chromatography, Coulometric detection, Amperometric detection, Heavy metal, Laser induced breakdown spectroscopy

Authors

Vaclav Diopan, Violetta Shestivska, Ondrej Zitka, Michaela Galiova, Vojtech Adam, Jozef Kaiser, Ales Horna, Karel Novotny, Miroslav Liska, Ladislav Havel, Josef Zehnalek, Rene Kizek

RIV year

2010

Released

12. 1. 2010

ISBN

1040-0397

Periodical

ELECTROANALYSIS

Year of study

22

Number

11

State

Federal Republic of Germany

Pages from

1248

Pages to

1259

Pages count

12

BibTex

@article{BUT49960,
  author="Jozef {Kaiser} and Miroslav {Liška} and Michaela {Vašinová Galiová}",
  title="Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions",
  journal="ELECTROANALYSIS",
  year="2010",
  volume="22",
  number="11",
  pages="1248--1259",
  issn="1040-0397"
}