Publication detail

Content of phenolic compounds and antioxidant capacity in fruits of selected genotypes of apricot with resistance against Plum pox virus

SOCHOR, J. ZÍTKA, O. ŠKUTKOVÁ, H. PAVLÍK, D. BABULA, P. KRŠKA, B. HORNA, A. ADAM, V. PROVAZNÍK, I. KIZEK, R.

Original Title

Content of phenolic compounds and antioxidant capacity in fruits of selected genotypes of apricot with resistance against Plum pox virus

English Title

Content of phenolic compounds and antioxidant capacity in fruits of selected genotypes of apricot with resistance against Plum pox virus

Type

journal article - other

Language

en

Original Abstract

Research on natural compounds is increasingly focused on their effects on human health. In this study, we were interested in the evaluation of nutritional value expressed as content of total phenolic compounds and antioxidant capacity of new apricot (Prunus armeniaca L.) genotypes resistant against Plum pox virus (PPV) cultivated on Department of Fruit Growing of Mendel University in Brno. Fruits of twenty one apricot genotypes were collected at the onset of consumption ripeness. Antioxidant capacities of the genotypes were determined spectrometrically using DPPH (1,1-diphenyl-2-picrylhydrazyl free radicals) scavenging test, TEAC (Trolox Equivalent Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power)methods. The highest antioxidant capacities were determined in the genotypes LE-3228 and LE-2527, the lowest ones in the LE-985 and LE-994 genotypes. Moreover, close correlation (r = 0.964) was determined between the TEAC and DPPH assays. Based on the antioxidant capacity and total polyphenols content, a clump analysis dendrogram of the monitored apricot genotypes was constructed. In addition, we optimized high performance liquid chromatography coupled with tandem electrochemical and spectrometric detection and determined phenolic profile consisting of the following fifteen phenolic compounds: gallic acid, 4-aminobenzoic acid, chlorogenic acid, ferulic acid, caffeic acid, procatechin, salicylic acid, p-coumaric acid, the flavonols quercetin and quercitrin, the flavonol glycoside rutin, resveratrol, vanillin, and the isomers epicatechin, (-)- and (+)- catechin.

English abstract

Research on natural compounds is increasingly focused on their effects on human health. In this study, we were interested in the evaluation of nutritional value expressed as content of total phenolic compounds and antioxidant capacity of new apricot (Prunus armeniaca L.) genotypes resistant against Plum pox virus (PPV) cultivated on Department of Fruit Growing of Mendel University in Brno. Fruits of twenty one apricot genotypes were collected at the onset of consumption ripeness. Antioxidant capacities of the genotypes were determined spectrometrically using DPPH (1,1-diphenyl-2-picrylhydrazyl free radicals) scavenging test, TEAC (Trolox Equivalent Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power)methods. The highest antioxidant capacities were determined in the genotypes LE-3228 and LE-2527, the lowest ones in the LE-985 and LE-994 genotypes. Moreover, close correlation (r = 0.964) was determined between the TEAC and DPPH assays. Based on the antioxidant capacity and total polyphenols content, a clump analysis dendrogram of the monitored apricot genotypes was constructed. In addition, we optimized high performance liquid chromatography coupled with tandem electrochemical and spectrometric detection and determined phenolic profile consisting of the following fifteen phenolic compounds: gallic acid, 4-aminobenzoic acid, chlorogenic acid, ferulic acid, caffeic acid, procatechin, salicylic acid, p-coumaric acid, the flavonols quercetin and quercitrin, the flavonol glycoside rutin, resveratrol, vanillin, and the isomers epicatechin, (-)- and (+)- catechin.

Keywords

apricot; DPPH; TEAC; FRAP; high performance liquid chromatography (HPLC); electrochemical and spectrometric detection; Plum pox virus (PPV); dendrogram

RIV year

2010

Released

07.09.2010

Publisher

MDPI

Pages from

6285

Pages to

6305

Pages count

21

URL

Full text in the Digital Library

BibTex


@article{BUT49817,
  author="Jiří {Sochor} and Ondřej {Zítka} and Helena {Škutková} and Dušan {Pavlík} and Petr {Babula} and Boris {Krška} and Aleš {Horna} and Vojtěch {Adam} and Ivo {Provazník} and René {Kizek}",
  title="Content of phenolic compounds and antioxidant capacity in fruits of selected genotypes of apricot with resistance against Plum pox virus",
  annote="Research on natural compounds is increasingly focused on their effects on human health. In this study, we were interested in the evaluation of nutritional value expressed as content of total phenolic compounds and antioxidant capacity of new apricot (Prunus armeniaca L.) genotypes resistant against Plum pox virus (PPV) cultivated on Department of Fruit Growing of Mendel University in Brno. Fruits of twenty one apricot genotypes were collected at the onset of consumption ripeness. Antioxidant capacities of the genotypes were determined spectrometrically using DPPH (1,1-diphenyl-2-picrylhydrazyl free radicals) scavenging test, TEAC (Trolox Equivalent Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power)methods. The highest antioxidant capacities were determined in the genotypes LE-3228 and LE-2527, the lowest ones in the LE-985 and LE-994 genotypes. Moreover, close correlation (r = 0.964) was determined between the TEAC and DPPH assays. Based on the antioxidant capacity and total polyphenols content, a clump analysis dendrogram of the monitored apricot genotypes was constructed. In addition, we optimized high performance liquid chromatography coupled with tandem electrochemical and spectrometric detection and determined phenolic profile consisting of the following fifteen phenolic compounds: gallic acid, 4-aminobenzoic acid, chlorogenic acid, ferulic acid, caffeic acid, procatechin, salicylic acid, p-coumaric acid, the flavonols quercetin and quercitrin, the flavonol glycoside rutin, resveratrol, vanillin, and the isomers epicatechin, (-)- and (+)- catechin.",
  address="MDPI",
  chapter="49817",
  doi="10.3390/molecules15096285",
  institution="MDPI",
  number="15",
  volume="2010",
  year="2010",
  month="september",
  pages="6285--6305",
  publisher="MDPI",
  type="journal article - other"
}