Publication detail

Miniaturized electrochemical detector as a tool for detection of DNA amplified by PCR

HÚSKA, D. HUBÁLEK, J. ADAM, V. KIZEK, R.

Original Title

Miniaturized electrochemical detector as a tool for detection of DNA amplified by PCR

Type

journal article - other

Language

English

Original Abstract

This paper reports on the analysis of specific sequence of Phage Lambda DNA amplified by PCR. Agarose gel electrophoresis, gel electrophoresis on chip and stationary electrochemical instrument were employed for detection of amplicons obtained after 2, 4, 6, 8, 10, 15, 20, 25, 30 and 35 cycles. In the case of agarose gel electrophoresis the lowest detectable amount of DNA was obtained after 15 PCR cycles. Gel electrophoresis on chip offers higher sensitivity because the lowest detectable amount of amplicons by this technique was obtained after eight PCR cycles. Further we employed square wave voltammetry and various working electrodes (hanging mercury drop electrode, screen-printed carbon electrode and carbon-nanotube-based screen-printed electrodes) to detect amplicons. Amplicons obtained even after two cycles were detectable at all electrodes. To improve the selectivity of electrochemical detection carbon nanoelectrodes were off-line coupled with gel electrophoresis. Into the agarose gel the electrodes were placed. Further the amplicons were loaded into agarose gel wells. DNA migrating to the detection place was electrochemically analyzed. Amplicons obtained after two cycles were detectable by this hyphenated technique.

Keywords

Carbon nanotubes; DNA; Gel electrophoresis on chip; PCR; Voltammetry

Key words in English

Carbon nanotubes; DNA; Gel electrophoresis on chip; PCR; Voltammetry

Authors

HÚSKA, D.; HUBÁLEK, J.; ADAM, V.; KIZEK, R.

RIV year

2009

Released

1. 1. 2009

Publisher

WILEY-V C H VERLAG GMBH

Location

PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY

ISBN

0173-0835

Periodical

Electrophoresis

Year of study

2009 (29)

Number

24

State

Federal Republic of Germany

Pages from

4964

Pages to

4971

Pages count

8

BibTex

@article{BUT48742,
  author="Dalibor {Húska} and Jaromír {Hubálek} and Vojtěch {Adam} and René {Kizek}",
  title="Miniaturized electrochemical detector as a tool for detection of DNA amplified by PCR",
  journal="Electrophoresis",
  year="2009",
  volume="2009 (29)",
  number="24",
  pages="4964--4971",
  issn="0173-0835"
}