Publication detail

Bio-Assessing of Environmental Pollution via Monitoring of Metallothionein Level Using Electrochemical Detection

STEJSKAL, K. KŘÍŽKOVÁ, S. ADAM, V. SURES, B. TRNKOVÁ, L. ZEHNÁLEK, J. HUBÁLEK, J. BEKLOVÁ, M. HANUSTIAK, P. SVOBODOVÁ, Z. HORNA, A. KIZEK, R.

Original Title

Bio-Assessing of Environmental Pollution via Monitoring of Metallothionein Level Using Electrochemical Detection

Type

journal article - other

Language

English

Original Abstract

In this paper, we report on an investigation of affecting guppy fishes (Poecilia reticulata) by silver ions (0, 0.3, 0.6, 1.2, 2.5, and 5 mu M) for seven days under well-controlled experimental conditions. To observe the physiological changes, we attempted to determine metallothionein (MT) as a biomarker of heavy metal stress. For this purpose, we proposed a sensor utilizing a carbon electrode coupled with flow injection analysis. The experimental conditions, which have been optimized, were as follows-applied potential: 750 mV, mobile phase: Britton-Robinson buffer (pH 1.9) with How rate of 0.6 ml/min, time filter: 2.5 s, "current R": 1 mu A. Under these conditions, the detection limit of MT was estimated as 100 pM. After the optimizing step, the fish tissues were measured. Based on the results obtained, MT content increased with increasing dose of silver ions and time of the treatment. The results obtained were in good correlation with those obtained by adsorptive transfer stripping technique coupled with differential pulse voltammetry Brdicka reaction, which was used as the reference technique.

Keywords

Amperometric detection; biomarker; electrochemical sensors; fish; flow injection analysis; heavy metal; metallothionein; poecilia reticulata; silver

Authors

STEJSKAL, K.; KŘÍŽKOVÁ, S.; ADAM, V.; SURES, B.; TRNKOVÁ, L.; ZEHNÁLEK, J.; HUBÁLEK, J.; BEKLOVÁ, M.; HANUSTIAK, P.; SVOBODOVÁ, Z.; HORNA, A.; KIZEK, R.

RIV year

2008

Released

1. 10. 2008

Publisher

IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC

Location

USA

ISBN

1530-437X

Periodical

IEEE SENSORS JOURNAL

Year of study

8

Number

9-10

State

United States of America

Pages from

1578

Pages to

1585

Pages count

8

BibTex

@article{BUT47213,
  author="STEJSKAL, K. and KŘÍŽKOVÁ, S. and ADAM, V. and SURES, B. and TRNKOVÁ, L. and ZEHNÁLEK, J. and HUBÁLEK, J. and BEKLOVÁ, M. and HANUSTIAK, P. and SVOBODOVÁ, Z. and HORNA, A. and KIZEK, R.",
  title="Bio-Assessing of Environmental Pollution via Monitoring of Metallothionein Level Using Electrochemical Detection",
  journal="IEEE SENSORS JOURNAL",
  year="2008",
  volume="8",
  number="9-10",
  pages="1578--1585",
  issn="1530-437X"
}