Publication detail

Electrochemical and spectrometric study of antioxidant activity of pomiferin, isopomiferin, osajin and catalposide

DIOPAN, V. BABULA, P. SHESTIVSKA, V. ADAM, V. ŽEMLIČKA, M. DVORSKÁ, M. HUBÁLEK, J. TRNKOVÁ, L. HAVEL, L. KIZEK, R.

Original Title

Electrochemical and spectrometric study of antioxidant activity of pomiferin, isopomiferin, osajin and catalposide

Type

journal article - other

Language

English

Original Abstract

The antioxidant properties of pomiferin, isopomiferin, osajin and catalposide are evaluated. The electrochemical behaviour of these compounds at a carbon paste electrode was studied using square wave voltammetry. Oxidative signals, optimized frequencies and appropriate pH acetate buffer conditionswere determined. The detection limits (3 S/N) for pomiferin, isopomiferin, osajin and catalposide were estimated to be 50 pg/ml, 800 pg/ml, 40 pg/ml and 10 ng/ml, respectively. Furthermore, spectrometric test was employed with 2,2-diphenyl-1-picrylhydrazyle (DPPH) to evaluate the antioxidant activities of these compounds. Based on the obtained results, the highest antioxidant activity measured by DPPH tests was found at pomiferin followed by isopomiferin. The activities of osajin and catalposide were undetectable. The protective effects of pomiferin, isopomiferin, osajin and catalposide on DNA exposed to oxygen radicals in vitrowere also studied.

Keywords

Flavonoids; Iridoid glucoside; DNA; Oxidation signal; Voltammetry; Antioxidant capacity

Authors

DIOPAN, V.; BABULA, P.; SHESTIVSKA, V.; ADAM, V.; ŽEMLIČKA, M.; DVORSKÁ, M.; HUBÁLEK, J.; TRNKOVÁ, L.; HAVEL, L.; KIZEK, R.

RIV year

2008

Released

28. 5. 2008

Publisher

ELSEVIER SCIENCE LTD

Location

OXFORD, ENGLAND

ISBN

0731-7085

Periodical

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Year of study

2008

Number

48

State

Kingdom of the Netherlands

Pages from

127

Pages to

133

Pages count

7

BibTex

@article{BUT47212,
  author="DIOPAN, V. and BABULA, P. and SHESTIVSKA, V. and ADAM, V. and ŽEMLIČKA, M. and DVORSKÁ, M. and HUBÁLEK, J. and TRNKOVÁ, L. and HAVEL, L. and KIZEK, R.",
  title="Electrochemical and spectrometric study of antioxidant activity of pomiferin, isopomiferin, osajin and catalposide",
  journal="JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS",
  year="2008",
  volume="2008",
  number="48",
  pages="127--133",
  issn="0731-7085"
}