Publication detail

Electrochemical biosensor for investigation of anticancer drugs interactions (doxorubicin and ellipticine) with DNA

TRNKOVÁ, L. STIBOROVÁ, M. HÚSKA, D. ADAM, V. KIZEK, R. HUBÁLEK, J.

Original Title

Electrochemical biosensor for investigation of anticancer drugs interactions (doxorubicin and ellipticine) with DNA

Type

conference paper

Language

English

Original Abstract

The mechanisms of action of chemotherapeutics are different, but very often interfere with processes of cell division and can bind onto DNA. The aim of this work was to investigate interactions of antitumour drugs (ellipticine and doxorubicin) with DNA. For these purposes our experiments the screen-printed nanotube carbon electrodes were used. DNA gives oxidation signals for all bases (adenine, cytosine, guanine and thymine). We introduced various ellipticine, doxorubicin and ethidium bromide concentrations to DNA on carbon electrodes fabricated by us and measured the changes in the height of nucleic acid bases signals. In the voltammograms measured the changes associated with the interactions of the target compounds with DNA were evident. The height of DNA signals decreased with increasing ellipticine, doxorubicin and ethidium bromide concentrations. The decrease can be related to changes of DNA structure caused by the intercalation.

Keywords

NEUROBLASTOMA-CELL LINES; ADDUCT FORMATION; NANOPARTICLES; CANCER; DEOXYGUANOSINE; MICROSOMES; RESISTANCE; OXIDATION; UPDATE; MODEL

Authors

TRNKOVÁ, L.; STIBOROVÁ, M.; HÚSKA, D.; ADAM, V.; KIZEK, R.; HUBÁLEK, J.

RIV year

2010

Released

25. 10. 2010

Publisher

IEEE

Location

New Zealand

ISBN

978-1-4244-4548-6

Book

2009 IEEE SENSORS, VOLS 1-3

Pages from

1146

Pages to

1149

Pages count

4

BibTex

@inproceedings{BUT33602,
  author="Libuše {Trnková} and Marie {Stiborová} and Dalibor {Húska} and Vojtěch {Adam} and René {Kizek} and Jaromír {Hubálek}",
  title="Electrochemical biosensor for investigation of anticancer drugs interactions (doxorubicin and ellipticine) with DNA",
  booktitle="2009 IEEE SENSORS, VOLS 1-3",
  year="2010",
  pages="1146--1149",
  publisher="IEEE",
  address="New Zealand",
  isbn="978-1-4244-4548-6"
}