Publication detail

Isolation of copolymers of polyhydroxyalkanoates (PHAs) from Aneurinibacillus sp. H1

SLANINOVÁ, E. KRUPIČKOVÁ, K. SEDLÁČEK, P. OBRUČA, S.

Original Title

Isolation of copolymers of polyhydroxyalkanoates (PHAs) from Aneurinibacillus sp. H1

Type

abstract

Language

English

Original Abstract

Polyhydroxyalkanoates (PHAs) are native polyesters accumulated as intracellular granules by numerous bacteria and the content of polymer reaches up to 90%wt of cell dry weight. These compounds are biodegradable and biocompatible which make them more suitable in comparison with conventional plastics. Especially copolymers have great potential due to better mechanical properties in comparison with homopolymers such as well known poly-3-hydroxybutyrate (P3HB). In our experiments, we used thermophilic isolate which was taxonomically classified as Aneurinibacillus sp. H1 as a PHA producing bacterial strain of capable of synthesis of copolymer P(3HB-co-4HB). For isolation of homopolymers of PHAs is mostly used chloroform. Using this solvent for extraction results in product which is still pure and without any physical changes. On other hand, chloroform does not among environmentally friendly solvents. Therefore, we used several alternative solvents such as acetone, isopropanol, tetrahydrofuran, ethylacetate to recover PHA from bacterial biomass. We used various techniques for characterization of efficiency of isolation, structural differences and molar mass of copolymer P(3HB-co-4HB) such as GC, ATR-FTIR, eSEM and SEC-MALS. Structural changes of films of isolated material were visible at a glance where number and size of microstructural pores were dependent on used solvent. This material with specific properties could be used in various branches of industry (food chemistry, medicine, etc.).

Keywords

polyhydroxyalkanoates; copolymer; Aneurinibacillus sp.; solvent

Authors

SLANINOVÁ, E.; KRUPIČKOVÁ, K.; SEDLÁČEK, P.; OBRUČA, S.

Released

25. 2. 2021

ISBN

1314-3530

Periodical

Biotechnology and Biotechnological Equipment

Year of study

35

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

20

Pages to

21

Pages count

1

BibTex

@misc{BUT169823,
  author="Eva {Slaninová} and Kristýna {Krupičková} and Petr {Sedláček} and Stanislav {Obruča}",
  title="Isolation of copolymers of polyhydroxyalkanoates (PHAs) from Aneurinibacillus sp. H1",
  year="2021",
  journal="Biotechnology and Biotechnological Equipment",
  volume="35",
  number="1",
  pages="20--21",
  issn="1314-3530",
  note="abstract"
}