Publication detail

A novel biocompatible titanium–gadolinium quantum dot as a bacterial detecting agent with high antibacterial activity

SUR, V. MAZUMDAR, A. ASHRAFI, A. MUKHERJEE, A. MILOSAVLJEVIĆ, V. MICHÁLKOVÁ, H. KOPEL, P. RICHTERA, L. MOULICK, A.

Original Title

A novel biocompatible titanium–gadolinium quantum dot as a bacterial detecting agent with high antibacterial activity

Type

journal article in Web of Science

Language

English

Original Abstract

In this study, the titanium gadolinium quantum dots (TGQDs) were novel, first of its type to be synthesized, and fully characterized to date. Multiple physical characterization includes scanning electron microscopy (SEM), scanning electrochemical microscope (SCEM), x-ray fluorescence, spectrophotometry, and dynamic light scattering were carried out. The obtained results confirmed appropriate size and shape distributions in addition to processing optical features with high quantum yield. The synthesized TGQD was used as a fluorescent dye for bacterial detection and imaging by fluorescent microscopy and spectrophotometry, where TGQD stained only bacterial cells, but not human cells. The significant antibacterial activities of the TGQDs were found against a highly pathogenic bacterium (Staphylococcus aureus) and its antibiotic resistant strains (vancomycin and methicillin resistant Staphylococcus aureus) using growth curve analysis and determination of minimum inhibitory concentration (MIC) analysis. Live/dead cell imaging assay using phase-contrast microscope was performed for further confirmation of the antibacterial activity. Cell wall disruption and release of cell content was observed to be the prime mode of action with the reduction of cellular oxygen demand (OD).

Keywords

bacterial resistance; titanium-gadolinium quantum dots; bacterial detection; antibacterial activity; SECM

Authors

SUR, V.; MAZUMDAR, A.; ASHRAFI, A.; MUKHERJEE, A.; MILOSAVLJEVIĆ, V.; MICHÁLKOVÁ, H.; KOPEL, P.; RICHTERA, L.; MOULICK, A.

Released

17. 4. 2020

Publisher

MDPI

ISBN

2079-4991

Periodical

Nanomaterials

Year of study

10

Number

4

State

Swiss Confederation

Pages from

1

Pages to

18

Pages count

18

URL

https://www.mdpi.com/2079-4991/10/4/778

Full text in the Digital Library

http://hdl.handle.net/11012/191379

BibTex

@article{BUT164383,
  author="Vishma Pratap {Sur} and Aninda {Mazumdar} and Amirmansoor {Ashrafi} and Atripan {Mukherjee} and Vedran {Milosavljević} and Hana {Michálková} and Pavel {Kopel} and Lukáš {Richtera} and Amitava {Moulick}",
  title="A novel biocompatible titanium–gadolinium quantum dot as a bacterial detecting agent with high antibacterial activity",
  journal="Nanomaterials",
  year="2020",
  volume="10",
  number="4",
  pages="1--18",
  doi="10.3390/nano10040778",
  issn="2079-4991",
  url="https://www.mdpi.com/2079-4991/10/4/778"
}