Publication detail

Influnce of Mutual Rotation of Polarizing Filters on Light Intensity Measured with Collagen Figres

TURČANOVÁ, M. HRTOŇ, M. DVOŘÁK, P. BURŠA, J.

Original Title

Influnce of Mutual Rotation of Polarizing Filters on Light Intensity Measured with Collagen Figres

English Title

Influnce of Mutual Rotation of Polarizing Filters on Light Intensity Measured with Collagen Figres

Type

book chapter

Language

en

Original Abstract

The most common technique for observation of collagen fibres and evaluation of their directionality is polarized light microscopy. In the microscope, there are two polarizing filters, polarizer and analyzer, that can rotate individually. The default position of the polarizer is perpendicular to the analyzer. When the light passes through a birefringent specimen, the plane of the polarized light is rotated. This leads to a 90◦ periodic change in light intensity passing through the sample and both filters. If the orientation of the polarizer differs from the perpendicular direction to the analyzer, the period of light intensity changes to 180◦; one of the original maxima increases and the other decreases. This change is very useful for the determination of directions of collagen fibres by an automatic algorithm.

English abstract

The most common technique for observation of collagen fibres and evaluation of their directionality is polarized light microscopy. In the microscope, there are two polarizing filters, polarizer and analyzer, that can rotate individually. The default position of the polarizer is perpendicular to the analyzer. When the light passes through a birefringent specimen, the plane of the polarized light is rotated. This leads to a 90◦ periodic change in light intensity passing through the sample and both filters. If the orientation of the polarizer differs from the perpendicular direction to the analyzer, the period of light intensity changes to 180◦; one of the original maxima increases and the other decreases. This change is very useful for the determination of directions of collagen fibres by an automatic algorithm.

Keywords

Polarized light microscopy; collagen fibers; birefrigence; light intensity

Released

28.09.2019

ISBN

978-3-030-32040-9

Book

VipIMAGE 2019

Pages from

279

Pages to

285

Pages count

7

Documents

BibTex


@inbook{BUT161919,
  author="Michaela {Turčanová} and Martin {Hrtoň} and Petr {Dvořák} and Jiří {Burša}",
  title="Influnce of Mutual Rotation of Polarizing Filters on Light Intensity Measured with Collagen Figres",
  annote="The most common technique for observation of collagen fibres and evaluation
of their directionality is polarized light microscopy. In the microscope, there
are two polarizing filters, polarizer and analyzer, that can rotate individually. The
default position of the polarizer is perpendicular to the analyzer. When the light
passes through a birefringent specimen, the plane of the polarized light is rotated.
This leads to a 90◦ periodic change in light intensity passing through the sample
and both filters. If the orientation of the polarizer differs from the perpendicular
direction to the analyzer, the period of light intensity changes to 180◦; one of the
original maxima increases and the other decreases. This change is very useful for
the determination of directions of collagen fibres by an automatic algorithm.",
  booktitle="VipIMAGE 2019
",
  chapter="161919",
  doi="10.1007/978-3-030-32040-9",
  howpublished="online",
  year="2019",
  month="september",
  pages="279--285",
  type="book chapter"
}