Publication detail

A simple technique for assessing the cuticular diffusion of humic acid biostimulants

SMILKOVÁ, M. SMILEK, J. KALINA, M. KLUČÁKOVÁ, M. PEKAŘ, M. SEDLÁČEK, P.

Original Title

A simple technique for assessing the cuticular diffusion of humic acid biostimulants

Type

journal article in Web of Science

Language

English

Original Abstract

Background: Experimental determination of the extent and rate of transport of liquid humates supplied to plants is critical in testing of physiological effects of such biostimulants which are often supplied as foliar sprays. Therefore, an original experimental method for the qualitative investigation and quantitative description of the penetration on humates through plant cuticles is proposed, tested, and evaluated. Results: The proposed method involves the isolation of model plant leaf cuticles and the subsequent in vitro evaluation of cuticular humate transport. The employed novel methodology is based on a simple diffusion couple arrangement involving continuous spectrophotometric determination of the amounts of penetrated humate in a hydrogel diffusion medium. Prunus laurocerasus leaf cuticles were isolated by chemical and enzymatic treatment and the rate of cuticular penetration of a commercial humate (lignohumate) was estimated over time in quantitative and qualitative terms. Different rates of lignohumate transport were determined for abaxial and adaxial leaf cuticles also in relation to the different cuticular extraction methods tested. Conclusions: The proposed methodology represents a simple and cheap experimental tool for the study on the trans-cuticular penetration of humic-based biostimulants.

Keywords

Diffusion, Hydrogel, Prunus laurocerasus, Liquid fertilization, Humic substances, Plant cuticle

Authors

SMILKOVÁ, M.; SMILEK, J.; KALINA, M.; KLUČÁKOVÁ, M.; PEKAŘ, M.; SEDLÁČEK, P.

Released

31. 7. 2019

Publisher

BMC

ISBN

1746-4811

Periodical

PLANT METHODS

Year of study

15

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

11

Pages count

11

URL

https://plantmethods.biomedcentral.com/articles/10.1186/s13007-019-0469-x#Abs1

Full text in the Digital Library

http://hdl.handle.net/11012/180811

BibTex

@article{BUT157782,
  author="Marcela {Smilková} and Jiří {Smilek} and Michal {Kalina} and Martina {Klučáková} and Miloslav {Pekař} and Petr {Sedláček}",
  title="A simple technique for assessing the cuticular diffusion of humic acid biostimulants",
  journal="PLANT METHODS",
  year="2019",
  volume="15",
  number="1",
  pages="1--11",
  doi="10.1186/s13007-019-0469-x",
  issn="1746-4811",
  url="https://plantmethods.biomedcentral.com/articles/10.1186/s13007-019-0469-x#Abs1"
}