Publication detail

Isolation of Arginine Using Maghemite Particles

CERNEI, N. KOPEL, P. HORKÝ, P. ZÍTKA, O. ADAM, V.

Original Title

Isolation of Arginine Using Maghemite Particles

Type

conference paper

Language

English

Original Abstract

In this paper, we report the optimal conditions for the isolation of amino acid arginine from different types of matrix, such as blood, plasma or tissue and their characterization using ion-exchange liquid chromatography with VIS detector. Paramagnetic microparticles able to isolate and immobilize amino acid arginine were prepared and used to preconcentrate arginine for subsequent analysis on ion-exchange liquid chromatography with VIS detector. Commercially available Dowex was covered by maghemite nanoparticles and the composite showed excellent binding and superparamagnetic properties. It was found that the paramagnetic microparticles were able to bind specifically amino acid arginine. Therefore, these paramagnetic microparticles have potential for better isolation of arginine and in future can use for application as a platform of delivery system.

Keywords

Ion exchange chromatography; maghemite microparticles; amino acids; arginine

Authors

CERNEI, N.; KOPEL, P.; HORKÝ, P.; ZÍTKA, O.; ADAM, V.

Released

1. 2. 2019

Publisher

TANGER

ISBN

978-80-87294-89-5

Book

NANOCON 2018

Pages from

210

Pages to

213

Pages count

4

URL

https://nanocon2018.tanger.cz/cz/

Full text in the Digital Library

http://hdl.handle.net/11012/178393

BibTex

@inproceedings{BUT156257,
  author="Natalia Vladimirovna {Cernei} and Pavel {Kopel} and Pavel {Horký} and Ondřej {Zítka} and Vojtěch {Adam}",
  title="Isolation of Arginine Using Maghemite Particles",
  booktitle="NANOCON 2018",
  year="2019",
  pages="210--213",
  publisher="TANGER",
  isbn="978-80-87294-89-5",
  url="https://nanocon2018.tanger.cz/cz/"
}