Publication detail

Investigating the interplay between sarcosine and Ca2+-dependent signaling in prostate cells

STRMISKA, V. BUCHTELOVÁ, H. MICHÁLEK, P. KŘÍŽKOVÁ, S. ADAM, V. HEGER, Z.

Original Title

Investigating the interplay between sarcosine and Ca2+-dependent signaling in prostate cells

Type

conference paper

Language

English

Original Abstract

It has been shown that sarcosine supplementation stimulates proliferation and invassivenes of prostate cells. Nevertheless, the exact molecular mechanism responsible for this phenomenon is not known. In the present study we demonstrate that sarcosine increases expression of calmodulin (CaM), an important intracellular signaling molecule. Through this, sarcosine activates calmodulin-dependent protein kinases signaling. Pathway of activation CaM-dependent protein kinases can activate regulation of mitosis, proliferation, cell death, gene transcription and phosphorylation/ dephosphorlation of proteins. This is done through CaM binding of four Ca2+ ions. Interestingly, in this study, we identified decrese in free Ca2+ correlating with sarcosine-induced up-regulation of CaM. The influence of CaM to cell cycle changes was further verified using post transcritrional gene silencing using CaM-siRNA complex. Co-treatment of prostate cells with CaM-siRNA and sarcosine showed decrease in CaM-dependent kinases and cell invasiveness compared to sarcosine treatment only.

Keywords

sarcosine; calmodulin; prostate; prostate cancer; human cells

Authors

STRMISKA, V.; BUCHTELOVÁ, H.; MICHÁLEK, P.; KŘÍŽKOVÁ, S.; ADAM, V.; HEGER, Z.

Released

2. 1. 2019

Publisher

Mendel University in Brno

ISBN

978-80-7509-597-8

Book

MendelNet 2018

Pages from

532

Pages to

536

Pages count

5

URL

https://mendelnet.cz/artkey/mnt-201801-0113_Investigating-the-interplay-between-sarcosine-and-Ca-sup-2-sup-dependent-signaling-in-prostate-cells.php?back=/magno/mnt/2018/mn1.php?secid=4

Full text in the Digital Library

http://hdl.handle.net/11012/178388

BibTex

@inproceedings{BUT156246,
  author="Vladislav {Strmiska} and Hana {Michálková} and Petr {Michálek} and Soňa {Křížková} and Vojtěch {Adam} and Zbyněk {Heger}",
  title="Investigating the interplay between sarcosine and Ca2+-dependent signaling in prostate cells",
  booktitle="MendelNet 2018",
  year="2019",
  pages="532--536",
  publisher="Mendel University in Brno",
  isbn="978-80-7509-597-8",
  url="https://mendelnet.cz/artkey/mnt-201801-0113_Investigating-the-interplay-between-sarcosine-and-Ca-sup-2-sup-dependent-signaling-in-prostate-cells.php?back=/magno/mnt/2018/mn1.php?secid=4"
}