Publication detail

Mass spectrometric imaging of cysteine rich proteins in human skin

POMPEIANO VANÍČKOVÁ, L. GURÁŇ, R. KOLLAR, S. EMRI, G. KŘÍŽKOVÁ, S. DO, T. HEGER, Z. ZÍTKA, O. ADAM, V.

Original Title

Mass spectrometric imaging of cysteine rich proteins in human skin

Type

journal article in Web of Science

Language

English

Original Abstract

Looking insight pathological processes, metallothioneins (MTs) are considered to be potential biomarkers for monitoring of a development of various types of diseases, such as cancer. The early identification of the MTs in biological tissues could be important tool for the estimation of appropriate clinical therapy. Therefore, here we investigated the application of matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) together with immunohistochemical analyses (IHC) using MT-1/2 antibody for MT detection in formalin-fixed paraffin-embedded (FFPE) biopsy specimens of human skin. Principal component analyses revealed differences in the peptide/protein profiles separating healthy skin from the carcinoma specimens. Statistically significant ion peaks at m/z 6038, 6300, 6676, and 7026 were more frequently detected in squamous cell carcinoma (SCC), basal cell carcinoma (BCC) and melanoma. Using IHC, we found that MT-1/2 was significantly higher in SCC and melanoma compared to healthy skin. Surprisingly, significantly low levels of MT-1/2 were found in BCC. On one side, the results indicate important role of MTs in melanoma occurrence and progression, as on the second side, there are hidden processes associated with MTs based on differences of the occurrence of the MS peaks, which could be associated with cycling of MTs isoforms.

Keywords

Metallothionein; Squamous cell carcinoma; Melanoma; MALDI MSI; Immunohistochemistry

Authors

POMPEIANO VANÍČKOVÁ, L.; GURÁŇ, R.; KOLLAR, S.; EMRI, G.; KŘÍŽKOVÁ, S.; DO, T.; HEGER, Z.; ZÍTKA, O.; ADAM, V.

Released

15. 3. 2019

Publisher

Elsevier

ISBN

0141-8130

Periodical

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Year of study

125

Number

1

State

Kingdom of the Netherlands

Pages from

270

Pages to

277

Pages count

8

URL

https://www.sciencedirect.com/science/article/pii/S0141813018356241

Full text in the Digital Library

http://hdl.handle.net/11012/194755

BibTex

@article{BUT156230,
  author="Lucie {Pompeiano Vaníčková} and Roman {Guráň} and Sandor {Kollar} and Gabriela {Emri} and Soňa {Křížková} and Tomáš {Do} and Zbyněk {Heger} and Ondřej {Zítka} and Vojtěch {Adam}",
  title="Mass spectrometric imaging of cysteine rich proteins in human skin",
  journal="INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES",
  year="2019",
  volume="125",
  number="1",
  pages="270--277",
  doi="10.1016/j.ijbiomac.2018.11.272",
  issn="0141-8130",
  url="https://www.sciencedirect.com/science/article/pii/S0141813018356241"
}