Publication detail

Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure

HORKÝ, P. SKALIČKOVÁ, S. URBÁNKOVÁ, L. BAHOLET, D. KOČIOVÁ, S. BYTEŠNÍKOVÁ, Z. KABOURKOVÁ, E. LACKOVÁ, Z. CERNEI, N. GAGIĆ, M. MILOSAVLJEVIĆ, V. SMOLÍKOVÁ, V. VÁCLAVKOVÁ, E. NEVRKLA, P. KNOT, P. KRYŠTOFOVÁ, O. HYNEK, D. KOPEL, P. SKLÁDANKA, J. ADAM, V. ŠMERKOVÁ, K.

Original Title

Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure

Type

journal article in Web of Science

Language

English

Original Abstract

Background: Development of new nanomaterials that inhibit or kill bacteria is an important and timely research topic. For example, financial losses due to infectious diseases, such as diarrhea, are a major concern in livestock productions around the world. Antimicrobial nanoparticles (NPs) represent a promising alternative to antibiotics and may lower antibiotic use and consequently spread of antibiotic resistance traits among bacteria, including pathogens. Results: Four formulations of zinc nanoparticles (ZnA, ZnB, ZnC, and ZnD) based on phosphates with spherical (ZnA, ZnB) or irregular (ZnC, ZnD) morphology were prepared. The highest in vitro inhibitory effect of our NPs was observed against Staphylococcus aureus (inhibitory concentration values, IC50, ranged from 0.5 to 1.6mmol/L), followed by Escherichia coli (IC50 0.8-1.5mmol/L). In contrast, methicillin resistant S. aureus (IC50 1.2-4.7mmol/L) was least affected and this was similar to inhibitory patterns of commercial ZnO-based NPs and ZnO. After the successful in vitro testing, the in vivo study with rats based on dietary supplementation with zinc NPs was conducted. Four groups of rats were treated by 2,000mg Zn/kg diet of ZnA, ZnB, ZnC, and ZnD, for comparison two groups were supplemented by 2,000mg Zn/kg diet of ZnO-N and ZnO, and one group (control) was fed only by basal diet. The significantly higher (P<0.05) Zn level in liver and kidney of all treated groups was found, nevertheless ZnNPs did not greatly influence antioxidant status of rats. However, the total aerobic and coliform bacterial population in rat feces significantly decreased (P<0.05) in all zinc groups after 30d of the treatment. Furthermore, when compared to the ZnO group, ZnA and ZnC nanoparticles reduced coliforms significantly more (P<0.05). Conclusions: Our results demonstrate that phosphate-based zinc nanoparticles have the potential to act as antibiotic agents.

Keywords

Aerobic bacteria; Antibiotics; Coliforms; Nanomaterials; Oxidative stress

Authors

HORKÝ, P.; SKALIČKOVÁ, S.; URBÁNKOVÁ, L.; BAHOLET, D.; KOČIOVÁ, S.; BYTEŠNÍKOVÁ, Z.; KABOURKOVÁ, E.; LACKOVÁ, Z.; CERNEI, N.; GAGIĆ, M.; MILOSAVLJEVIĆ, V.; SMOLÍKOVÁ, V.; VÁCLAVKOVÁ, E.; NEVRKLA, P.; KNOT, P.; KRYŠTOFOVÁ, O.; HYNEK, D.; KOPEL, P.; SKLÁDANKA, J.; ADAM, V.; ŠMERKOVÁ, K.

Released

12. 2. 2019

Publisher

BioMed Central

ISBN

2049-1891

Periodical

Journal of Animal Science and Biotechnology

Year of study

10

Number

1

State

People's Republic of China

Pages from

1

Pages to

12

Pages count

12

URL

http://link.springer.com/article/10.1186/s40104-019-0319-8

Full text in the Digital Library

http://hdl.handle.net/11012/138317

BibTex

@article{BUT156204,
  author="Pavel {Horký} and Sylvie {Skaličková} and Lenka {Urbánková} and Daria {Baholet} and Silvia {Kočiová} and Zuzana {Bytešníková} and Eliška {Kabourková} and Zuzana {Lacková} and Natalia Vladimirovna {Cernei} and Milica {Gagić} and Vedran {Milosavljević} and Vendula {Smolíková} and Eva {Václavková} and Pavel {Nevrkla} and Pavel {Knot} and Olga {Kryštofová} and David {Hynek} and Pavel {Kopel} and Jiří {Skládanka} and Vojtěch {Adam} and Kristýna {Šmerková}",
  title="Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure",
  journal="Journal of Animal Science and Biotechnology",
  year="2019",
  volume="10",
  number="1",
  pages="1--12",
  doi="10.1186/s40104-019-0319-8",
  issn="2049-1891",
  url="http://link.springer.com/article/10.1186/s40104-019-0319-8"
}