Publication detail

Separation of Nucleobases Using High-performance Liquid Chromatography Coupled with Voltammetric Scanning

ZÍTKA, O. KUDR, J. LABUDA, J. XHAXHIU, K. ADAM, V.

Original Title

Separation of Nucleobases Using High-performance Liquid Chromatography Coupled with Voltammetric Scanning

Type

journal article in Web of Science

Language

English

Original Abstract

In this paper, a method based on chromatographic separation of analytes and their quantification using on-line cyclic voltammetry is reported. The method based on high performance liquid chromatography on reverse phase column was optimized using free nucleobases-guanine, adenine, thymine, and cytosine. The optimal separation of nucleobases was detected when using 0.05M borate buffer (pH9.0) as the mobile phase, at isocratic flow rate 1mLmin(-1), and at separation column temperature of 30 degrees C. The accurate timing of the cyclic voltammetry enabled us to quantify the concentrations of individual nucleobases by oxidation on glassy carbon electrode.

Keywords

Glassy carbon electrode; On-line voltammetry scan; Nucleic acid; Nucleobases; Reverse phase

Authors

ZÍTKA, O.; KUDR, J.; LABUDA, J.; XHAXHIU, K.; ADAM, V.

Released

31. 8. 2018

ISBN

1040-0397

Periodical

ELECTROANALYSIS

Year of study

30

Number

8

State

Federal Republic of Germany

Pages from

1857

Pages to

1861

Pages count

5

BibTex

@article{BUT149617,
  author="Ondřej {Zítka} and Jiří {Kudr} and Ján {Labuda} and Kledi {Xhaxhiu} and Vojtěch {Adam}",
  title="Separation of Nucleobases Using High-performance Liquid Chromatography Coupled with Voltammetric Scanning",
  journal="ELECTROANALYSIS",
  year="2018",
  volume="30",
  number="8",
  pages="1857--1861",
  doi="10.1002/elan.201800175",
  issn="1040-0397"
}