Publication detail

Biocompatibility and regenerative abilities of resorbable nanostructured bilayer skin replacement evaluated by small and large animal model

VOJTOVÁ, L. BABRNÁKOVÁ, J. PAVLIŇÁKOVÁ, V. VIŠTEJNOVÁ, L. ŠTĚPÁNKOVÁ, V. FILOVA, E. BLAHNOVÁ, V. KNOZ, M. LIPOVÝ, B. HOLOUBEK, J. PAVLOVSKÝ, Z. FALDYNA, M. GÖPFERT, E. DAMBORSKÝ, J. HEARNDEN, V.

Original Title

Biocompatibility and regenerative abilities of resorbable nanostructured bilayer skin replacement evaluated by small and large animal model

English Title

Biocompatibility and regenerative abilities of resorbable nanostructured bilayer skin replacement evaluated by small and large animal model

Type

conference proceedings

Language

en

Original Abstract

The resorbable dermal replacement was made up of two nanostructured biocompatible layers. The lower part (dermis substitution) was prepared from biopolymer porous foam modified with a highly stable fibroblast growth factor 2 (FGF2). The upper part (dermo - epidermal substitution) consisted of a layer of amphiphilic polymeric nanofibers ensuring optimal adhesion to the epidermal graft. The effect of FGF2 concentration on morphology and biological activity of the skin substitutes was optimized in-vitro by colonization of 3T3 line of mouse fibroblasts. As confirmed by Chick Chorioallantoic Membrane assay, stable FGF2 also significantly enhanced neovascularization efficiency of biopolymer substitutes. Regenerative ability of the resorbable bilayer implant enriched with FGF2 was evaluated in a full-skin thickness defect on both small and large animal model (New Zealand rabbit and Large white pig, respectively). Histological and immuno-histological analysis of samples taken on the 14th postoperative day from rabbit defects confirmed the resorption of the biopolymeric bilayer and wound healing in the entire defect with the positive effect of FGF2. In both rabbits and pigs, the bilayer skin replacements showed the formation of new connective tissue without immune cells infiltration. Samples having incorporated FGF2 exhibited better elasticity of pig’s healed dermo-epidermal graft measured by cutometer. The tested 3D bilayer biomaterial has proven optimal properties for application to skin defects and wounds, shelf life under sterile conditions, resistance to infection, biocompatibility with proliferative and healing potential, and has the potential for the use in human medicine and clinical practice.

English abstract

The resorbable dermal replacement was made up of two nanostructured biocompatible layers. The lower part (dermis substitution) was prepared from biopolymer porous foam modified with a highly stable fibroblast growth factor 2 (FGF2). The upper part (dermo - epidermal substitution) consisted of a layer of amphiphilic polymeric nanofibers ensuring optimal adhesion to the epidermal graft. The effect of FGF2 concentration on morphology and biological activity of the skin substitutes was optimized in-vitro by colonization of 3T3 line of mouse fibroblasts. As confirmed by Chick Chorioallantoic Membrane assay, stable FGF2 also significantly enhanced neovascularization efficiency of biopolymer substitutes. Regenerative ability of the resorbable bilayer implant enriched with FGF2 was evaluated in a full-skin thickness defect on both small and large animal model (New Zealand rabbit and Large white pig, respectively). Histological and immuno-histological analysis of samples taken on the 14th postoperative day from rabbit defects confirmed the resorption of the biopolymeric bilayer and wound healing in the entire defect with the positive effect of FGF2. In both rabbits and pigs, the bilayer skin replacements showed the formation of new connective tissue without immune cells infiltration. Samples having incorporated FGF2 exhibited better elasticity of pig’s healed dermo-epidermal graft measured by cutometer. The tested 3D bilayer biomaterial has proven optimal properties for application to skin defects and wounds, shelf life under sterile conditions, resistance to infection, biocompatibility with proliferative and healing potential, and has the potential for the use in human medicine and clinical practice.

Keywords

skin replacement, dermal replacement, FGF2, fibroblast growth factor, biopolymer, dermis substitution, skin defect

Released

23.07.2018

Publisher

Scientific Federation

Location

Barcelona

Pages from

23

Pages to

23

Pages count

1

BibTex


@proceedings{BUT149214,
  author="Lucy {Vojtová} and Johana {Muchová} and Veronika {Pavliňáková} and Lucie {Vištejnová} and Veronika {Štěpánková} and Eva {Filova} and Veronika {Blahnová} and Martin {Knoz} and Břetislav {Lipový} and Jakub {Holoubek} and Zdeněk {Pavlovský} and Martin {Faldyna} and Eduard {Göpfert} and Jiří {Damborský} and Vanessa {Hearnden}",
  title="Biocompatibility and regenerative abilities of resorbable nanostructured bilayer skin replacement evaluated by small and large animal model",
  annote="The resorbable dermal replacement was made up of two nanostructured biocompatible layers. The lower part (dermis substitution) was prepared from biopolymer porous foam modified with a highly stable fibroblast growth factor 2 (FGF2). The upper part (dermo - epidermal substitution) consisted of a layer of amphiphilic polymeric nanofibers ensuring optimal adhesion to the epidermal graft. The effect of FGF2 concentration on morphology and biological activity of the skin substitutes was optimized in-vitro by colonization of 3T3 line of mouse fibroblasts. As confirmed by Chick Chorioallantoic Membrane assay, stable FGF2 also significantly enhanced neovascularization efficiency of biopolymer substitutes. Regenerative ability of the resorbable bilayer implant enriched with FGF2 was evaluated in a full-skin thickness defect on both small and large animal model (New Zealand rabbit and Large white pig, respectively). Histological and immuno-histological analysis of samples taken on the 14th postoperative day from rabbit defects confirmed the resorption of the biopolymeric bilayer and wound healing in the entire defect with the positive effect of FGF2. In both rabbits and pigs, the bilayer skin replacements showed the formation of new connective tissue without immune cells infiltration. Samples having incorporated FGF2 exhibited better elasticity of pig’s healed dermo-epidermal graft measured by cutometer. The tested 3D bilayer biomaterial has proven optimal properties for application to skin defects and wounds, shelf life under sterile conditions, resistance to infection, biocompatibility with proliferative and healing potential, and has the potential for the use in human medicine and clinical practice.",
  address="Scientific Federation",
  chapter="149214",
  howpublished="print",
  institution="Scientific Federation",
  year="2018",
  month="july",
  pages="23--23",
  publisher="Scientific Federation",
  type="conference proceedings"
}