Publication detail

Molecular imprinting technology for targeted analysis of proteins

HUTAŘOVÁ, J. VANĚČKOVÁ, T. VACULOVIČOVÁ, M. ADAM, V.

Original Title

Molecular imprinting technology for targeted analysis of proteins

Type

conference paper

Language

English

Original Abstract

Molecular imprinting has appeared to be an effective technique for creating of selective recognition sites in synthetic polymers. This procedure comprises polymerization of monomer in a presence of target molecules (template). The subsequent template removal forms tailor-made cavities that are complementary in shape and size to the template molecules. For protein imprinting, the choice of the suitable polymers is limited and polymerization conditions need to be optimized. In our work, dopamine monomer was chosen for polymer formation due to its nontoxicity, ease of preparation and self-assembly. For the optimization of conditions, lysozyme with molecular weight 14.3 kDa was used and the functionality was evaluated by fluorimetry. Different concentration of dopamine and lysozyme for polymerization were tested. Under the optimized conditions, the limit of detection for lysozyme was found to be 7.8 µg/ml. Moreover, conditions for polymer formation for a purpose to reduce the overall time of analysis were investigated. The use of dopamine as a monomer in molecular imprinting shown to be beneficial in many aspects.

Keywords

Polydopamine; molecularly imprinted polymer; lysozyme

Authors

HUTAŘOVÁ, J.; VANĚČKOVÁ, T.; VACULOVIČOVÁ, M.; ADAM, V.

Released

31. 12. 2017

Publisher

Mendel University in Brno

ISBN

978-80-7509-529-9

Book

MendelNet 2017

Pages from

873

Pages to

877

Pages count

5

URL

https://mendelnet.cz/artkey/mnt-201701-0098_Molecular-imprinting-technology-for-targeted-analysis-of-proteins.php?back=/magno/mnt/2017/mn1.php?secid=4

Full text in the Digital Library

http://hdl.handle.net/11012/84189

BibTex

@inproceedings{BUT148729,
  author="Jitka {Hutařová} and Tereza {Vaněčková} and Markéta {Vaculovičová} and Vojtěch {Adam}",
  title="Molecular imprinting technology for targeted analysis of proteins",
  booktitle="MendelNet 2017",
  year="2017",
  pages="873--877",
  publisher="Mendel University in Brno",
  isbn="978-80-7509-529-9",
  url="https://mendelnet.cz/artkey/mnt-201701-0098_Molecular-imprinting-technology-for-targeted-analysis-of-proteins.php?back=/magno/mnt/2017/mn1.php?secid=4"
}