Publication detail

Isolation of reduced and oxidized glutathione using maghemite particles

CERNEI, N. KOPEL, P. MILOSAVLJEVIĆ, V. HORKÝ, P. ADAM, V.

Original Title

Isolation of reduced and oxidized glutathione using maghemite particles

Type

conference paper

Language

English

Original Abstract

Here we report the optimal conditions for the isolation of reduced glutathione (GSH) and oxidized glutathione (GSSG) from various types of matrix, such as blood, plasma or cells and their characterization using ionexchange liquid chromatography with VIS detector. In our study, we aimed to synthesize paramagnetic microparticles able to isolate and immobilize GSH, GSSG from blood or plasma and thus preconcentrate it for subsequent analysis on ion-exchange liquid chromatography with VIS detector. We modified amberlite and Dowex by nanomaghemite (γ-Fe2O3), as the functional carriers providing perfect paramagnetic properties. Both the paramagnetic microparticles were able to bind GSH and GSSG. Our paramagnetic microparticles have potential to improve GSH and GSSG isolation and in future can serve for application as a platform of biosensors or delivery system.

Keywords

Ion exchange chromatography; maghemite microparticles; glutathione; reactive oxygen species

Authors

CERNEI, N.; KOPEL, P.; MILOSAVLJEVIĆ, V.; HORKÝ, P.; ADAM, V.

Released

31. 3. 2018

ISBN

978-80-87294-81-9

Book

NANOCON 2017

Edition

1st Edition, 2018

Pages from

165

Pages to

169

Pages count

5

BibTex

@inproceedings{BUT148688,
  author="Natalia Vladimirovna {Cernei} and Pavel {Kopel} and Vedran {Milosavljević} and Pavel {Horký} and Vojtěch {Adam}",
  title="Isolation of reduced and oxidized glutathione using maghemite particles",
  booktitle="NANOCON 2017",
  year="2018",
  series="1st Edition, 2018",
  pages="165--169",
  isbn="978-80-87294-81-9"
}