Publication detail

Modern semi-automatic set-up for testing cell migration with impact for therapy of myocardial infarction

BAIAZITOVA, L. SKOPALÍK, J. ČMIEL, V. CHMELÍK, J. SVOBODA, O. PROVAZNÍK, I.

Original Title

Modern semi-automatic set-up for testing cell migration with impact for therapy of myocardial infarction

English Title

Modern semi-automatic set-up for testing cell migration with impact for therapy of myocardial infarction

Type

conference paper

Language

en

Original Abstract

Ischemic heart disease and resulting acute myocardial infarction (AMI) is one of the main causes of morbidity and mortality in industrial countries. The idea for the modern therapeutic strategy, which should activate the migration of stem/progenitor cells or reduce the migration of inflammatory cells in AMI re-gions, has emerged in the last 15 years, mainly as a result of physiological obser-vation and post-mortem histology. Published data from direct measurements of cell migration are very limited. We prepared a universal set-up that can be used for the testing of cell migration in AMI micro-environment. Mesenchymal stro-mal cells (MSCs), the most commonly used stem/progenitor cells in experimental cellular therapy for AMI, were used in the recent set-up tests. The cells, which should be tested for their migration potential, were injected into the starting point in a special micro-chamber on the substrate, and optics of the microscope allowed a time-lapse recording of cells in micrometre resolution every two minutes. Our software tools provided precise 2D and 3D tracking of moving cells and data ex-port for statistical analysis. Set-up should be upgraded to a fully-automatic pre-clinical screening tool in the future.

English abstract

Ischemic heart disease and resulting acute myocardial infarction (AMI) is one of the main causes of morbidity and mortality in industrial countries. The idea for the modern therapeutic strategy, which should activate the migration of stem/progenitor cells or reduce the migration of inflammatory cells in AMI re-gions, has emerged in the last 15 years, mainly as a result of physiological obser-vation and post-mortem histology. Published data from direct measurements of cell migration are very limited. We prepared a universal set-up that can be used for the testing of cell migration in AMI micro-environment. Mesenchymal stro-mal cells (MSCs), the most commonly used stem/progenitor cells in experimental cellular therapy for AMI, were used in the recent set-up tests. The cells, which should be tested for their migration potential, were injected into the starting point in a special micro-chamber on the substrate, and optics of the microscope allowed a time-lapse recording of cells in micrometre resolution every two minutes. Our software tools provided precise 2D and 3D tracking of moving cells and data ex-port for statistical analysis. Set-up should be upgraded to a fully-automatic pre-clinical screening tool in the future.

Keywords

Patient screening, stem cell analysis, cell motility, time-lapse image analysis.

Released

30.05.2018

Publisher

Springer

Location

Singapore

ISBN

978-981-10-9022-6

Book

World Congress on Medical Physics and Biomedical Engineering 2018

Pages from

155

Pages to

159

Pages count

5

URL

BibTex


@inproceedings{BUT148130,
  author="Larisa {Baiazitova} and Josef {Skopalík} and Vratislav {Čmiel} and Jiří {Chmelík} and Ondřej {Svoboda} and Ivo {Provazník}",
  title="Modern semi-automatic set-up for testing cell migration with impact for therapy of myocardial infarction",
  annote="Ischemic heart disease and resulting acute myocardial infarction (AMI) is one of the main causes of morbidity and mortality in industrial countries. The idea for the modern therapeutic strategy, which should activate the migration of stem/progenitor cells or reduce the migration of inflammatory cells in AMI re-gions, has emerged in the last 15 years, mainly as a result of physiological obser-vation and post-mortem histology. Published data from direct measurements of cell migration are very limited. We prepared a universal set-up that can be used for the testing of cell migration in AMI micro-environment. Mesenchymal stro-mal cells (MSCs), the most commonly used stem/progenitor cells in experimental cellular therapy for AMI, were used in the recent set-up tests. The cells, which should be tested for their migration potential, were injected into the starting point in a special micro-chamber on the substrate, and optics of the microscope allowed a time-lapse recording of cells in micrometre resolution every two minutes. Our software tools provided precise 2D and 3D tracking of moving cells and data ex-port for statistical analysis. Set-up should be upgraded to a fully-automatic pre-clinical screening tool in the future.",
  address="Springer",
  booktitle="World Congress on Medical Physics and Biomedical Engineering 2018",
  chapter="148130",
  doi="10.1007/978-981-10-9023-3_28",
  howpublished="online",
  institution="Springer",
  number="3",
  year="2018",
  month="may",
  pages="155--159",
  publisher="Springer",
  type="conference paper"
}