Publication detail

Soluble collagen dissolution and assembling in pressurized carbon dioxide water solutions

ZUBAL, L. BONANI, W. MANIGLIO, D. CECCATO, R. RENČIUK, D. HAMPL, A. MIGLIARESI, C. JANČÁŘ, J. VOJTOVÁ, L.

Original Title

Soluble collagen dissolution and assembling in pressurized carbon dioxide water solutions

English Title

Soluble collagen dissolution and assembling in pressurized carbon dioxide water solutions

Type

journal article in Web of Science

Language

en

Original Abstract

Dissolution and gelation procedures have a great influence on gelation time, microstructure and mechanical properties of reconstituted collagen products. We have investigated the dissolution of atelocollagen in CO2/water solutions at low temperature (4 degrees C) at different CO2 pressures (0.3-0.9 MPa), as well as gelation kinetics and physico-chemical properties of the hydrogel obtained after CO2 removal. Compared to conventional methods, the CO2-assisted technique resulted in faster soluble collagen dissolution and faster gelation into transparent gels characterized by thin 10 nm fibrils. Electrophoresis and CD spectroscopy demonstrated that the process did not denature the soluble collagen. The possibility to obtain collagen dissolution and gelation without the use of chemical agent other than water and CO2 makes this process particularly appealing for biomedical applications.

English abstract

Dissolution and gelation procedures have a great influence on gelation time, microstructure and mechanical properties of reconstituted collagen products. We have investigated the dissolution of atelocollagen in CO2/water solutions at low temperature (4 degrees C) at different CO2 pressures (0.3-0.9 MPa), as well as gelation kinetics and physico-chemical properties of the hydrogel obtained after CO2 removal. Compared to conventional methods, the CO2-assisted technique resulted in faster soluble collagen dissolution and faster gelation into transparent gels characterized by thin 10 nm fibrils. Electrophoresis and CD spectroscopy demonstrated that the process did not denature the soluble collagen. The possibility to obtain collagen dissolution and gelation without the use of chemical agent other than water and CO2 makes this process particularly appealing for biomedical applications.

Keywords

biocompatible polymers; collagen fibrillogenesis; structural analysis; viscoelastic properties; protein self-assembly

Released

01.02.2018

Publisher

Budapest University of Technology

Pages from

159

Pages to

170

Pages count

12

URL

Full text in the Digital Library

BibTex


@article{BUT146700,
  author="Lukáš {Zubal} and Walter {Bonani} and Devid {Maniglio} and Riccardo {Ceccato} and Daniel {Renčiuk} and Aleš {Hampl} and Claudio {Migliaresi} and Josef {Jančář} and Lucy {Vojtová}",
  title="Soluble collagen dissolution and assembling in pressurized carbon dioxide water solutions",
  annote="Dissolution and gelation procedures have a great influence on gelation time, microstructure and mechanical properties of reconstituted collagen products. We have investigated the dissolution of atelocollagen in CO2/water solutions at low temperature (4 degrees C) at different CO2 pressures (0.3-0.9 MPa), as well as gelation kinetics and physico-chemical properties of the hydrogel obtained after CO2 removal. Compared to conventional methods, the CO2-assisted technique resulted in faster soluble collagen dissolution and faster gelation into transparent gels characterized by thin 10 nm fibrils. Electrophoresis and CD spectroscopy demonstrated that the process did not denature the soluble collagen. The possibility to obtain collagen dissolution and gelation without the use of chemical agent other than water and CO2 makes this process particularly appealing for biomedical applications.",
  address="Budapest University of Technology",
  chapter="146700",
  doi="10.3144/expresspolymlett.2018.14",
  howpublished="print",
  institution="Budapest University of Technology",
  number="2",
  volume="12",
  year="2018",
  month="february",
  pages="159--170",
  publisher="Budapest University of Technology",
  type="journal article in Web of Science"
}