MICRO RNA-124 DETERMINATION EMPLOYING MAGNETIC PARTICLES AND QUANTUM DOTS

conference paper

English

MicroRNAs (miRNAs) are a novel class of evolutionary conserved single-stranded RNAs, which have an important role in the regulation of gene expression at the posttranscriptional level. An effect of miRNAs is most often based on the binding to the untranslated region (3’UTR) of target mRNA causing degradation (or inhibition) of target mRNA. It is not surprising that they influence numerous cellular processes such as proliferation, differentiation, apoptosis, metastases, angiogenesis, and immune response [1, 2], of these many are connected with diseases including tumor ones. Recently, many studies have shown that the miRNAs profile may vary in numerous types of cancers like breast cancer [3, 4], hepatocellular carcinoma [5], lymphoblastic leukemia [6] and prostate cancer [7]. Specific miRNAs also contribute 396 to neural differentiation of neurons [8], regulate proliferation [9] and gastrulation of stem cells [10]. In this study, we coupled magnetic particles-based extraction with fluorescent labeling by streptavidin-coated CdTe quantum dots and capillary electrophoretic analysis with laser-induced fluorescent detection for detection of miRNA.

MicroRNAs; quantum dots

ŠMERKOVÁ, K.; STANISAVLJEVIC, M.; BLAŽKOVÁ, I.; VACULOVIČOVÁ, M.; ADAM, V.; KIZEK, R.

22. 10. 2014

978-80-904959-2-0

CECE 2014

396

399

4