Publication detail

Tularemia Progression Accompanied with Oxidative Stress and Antioxidant Alteration in Spleen and Liver of BALB/c Mice

POHANKA, M. PAVLIS, O. RUTTKAY-NEDECKÝ, B. SOCHOR, J. SOBOTKA, J. PIKULA, J. ADAM, V. KIZEK, R.

Original Title

Tularemia Progression Accompanied with Oxidative Stress and Antioxidant Alteration in Spleen and Liver of BALB/c Mice

Type

journal article in Web of Science

Language

English

Original Abstract

Francisella tularensis is the causative agent of tularemia. It is an intracellular pathogen with the ability to survive within phagosomes and induce pyroptotic cell death. In this study, we attempted to prove whether oxidative imbalance plays a significant role in tularemia pathogenesis. In our experimental model, we subcutaneously infected female BALB/c mice (dose 10(5) CFU of F. tularensis LVS). Liver, spleen, and blood were collected from mice at regular intervals from days 1-15 after infection. The bacterial burden was assessed by a cultivation test. The burden was unchanging from the 2nd to 6th day after infection. The bacterial burden corresponded to the plasmatic level of IFN-gamma, IL-6, and liver malondialdehyde. After the phase of acute bacteraemia and the innate immunity reaction, the levels of reduced glutathione and total low molecular weight antioxidants decreased significantly and the activity of caspase-3 increased in the liver. The level of reduced glutathione decreased to 25% of the original level, and the total level of low molecular weight antioxidants was less than 50% of the initial amount. The demonstrated effects of tularemia-induced pathology had a more extensive impact on the liver than on the spleen.

Keywords

Francisella tularensis; tularaemia; oxidative stress; inflammation; antioxidant; glutathione

Authors

POHANKA, M.; PAVLIS, O.; RUTTKAY-NEDECKÝ, B.; SOCHOR, J.; SOBOTKA, J.; PIKULA, J.; ADAM, V.; KIZEK, R.

Released

1. 6. 2012

ISBN

1225-8873

Periodical

JOURNAL OF MICROBIOLOGY

Year of study

50

Number

3

State

Republic of Korea

Pages from

401

Pages to

408

Pages count

8

BibTex

@article{BUT145191,
  author="POHANKA, M. and PAVLIS, O. and RUTTKAY-NEDECKÝ, B. and SOCHOR, J. and SOBOTKA, J. and PIKULA, J. and ADAM, V. and KIZEK, R.",
  title="Tularemia Progression Accompanied with Oxidative Stress and Antioxidant Alteration in Spleen and Liver of BALB/c Mice",
  journal="JOURNAL OF MICROBIOLOGY",
  year="2012",
  volume="50",
  number="3",
  pages="401--408",
  doi="10.1007/s12275-012-1621-8",
  issn="1225-8873"
}