Publication detail

A Novel Insight into the Cardiotoxicity of Antineoplastic Drug Doxorubicin

HEGER, Z. CERNEI, N. KUDR, J. GUMULEC, J. BLAŽKOVÁ, I. ZÍTKA, O. ECKSCHLAGER, T. STIBOROVÁ, M. ADAM, V. KIZEK, R.

Original Title

A Novel Insight into the Cardiotoxicity of Antineoplastic Drug Doxorubicin

Type

journal article in Web of Science

Language

English

Original Abstract

Doxorubicin is a commonly used antineoplastic agent in the treatment of many types of cancer. Little is known about the interactions of doxorubicin with cardiac biomolecules. Serious cardiotoxicity including dilated cardiomyopathy often resulting in a fatal congestive heart failure may occur as a consequence of chemotherapy with doxorubicin. The purpose of this study was to determine the effect of exposure to doxorubicin on the changes in major amino acids in tissue of cardiac muscle (proline, taurine, glutamic acid, arginine, aspartic acid, leucine, glycine, valine, alanine, isoleucine, threonine, lysine and serine). An in vitro interaction study was performed as a comparison of amino acid profiles in heart tissue before and after application of doxorubicin. We found that doxorubicin directly influences myocardial amino acid representation even at low concentrations. In addition, we performed an interaction study that resulted in the determination of breaking points for each of analyzed amino acids. Lysine, arginine, -alanine, valine and serine were determined as the most sensitive amino acids. Additionally we compared amino acid profiles of myocardium before and after exposure to doxorubicin. The amount of amino acids after interaction with doxorubicin was significantly reduced (p = 0.05). This fact points at an ability of doxorubicin to induce changes in quantitative composition of amino acids in myocardium. Moreover, this confirms that the interactions between doxorubicin and amino acids may act as another factor most likely responsible for adverse effects of doxorubicin on myocardium.

Keywords

myocardium; cardiomyopathy; interaction; amide bond; spectrophotometry; ion-exchange liquid chromatography

Authors

HEGER, Z.; CERNEI, N.; KUDR, J.; GUMULEC, J.; BLAŽKOVÁ, I.; ZÍTKA, O.; ECKSCHLAGER, T.; STIBOROVÁ, M.; ADAM, V.; KIZEK, R.

Released

1. 11. 2013

Publisher

MDPI

ISBN

1422-0067

Periodical

International Journal of Molecular Sciences

Year of study

14

Number

11

State

Swiss Confederation

Pages from

21629

Pages to

21646

Pages count

18

URL

https://www.mdpi.com/1422-0067/14/11/21629

Full text in the Digital Library

http://hdl.handle.net/11012/137222

BibTex

@article{BUT145130,
  author="Zbyněk {Heger} and Natalia Vladimirovna {Cernei} and Jiří {Kudr} and Jaromír {Gumulec} and Iva {Blažková} and Ondřej {Zítka} and Tomáš {Eckschlager} and Marie {Stiborová} and Vojtěch {Adam} and René {Kizek}",
  title="A Novel Insight into the Cardiotoxicity of Antineoplastic Drug Doxorubicin",
  journal="International Journal of Molecular Sciences",
  year="2013",
  volume="14",
  number="11",
  pages="21629--21646",
  doi="10.3390/ijms141121629",
  issn="1422-0067",
  url="https://www.mdpi.com/1422-0067/14/11/21629"
}