Publication detail

An Acetylcholinesterase-Based Chronoamperometric Biosensor for Fast and Reliable Assay of Nerve Agents

POHANKA, M. ADAM, V. KIZEK, R.

Original Title

An Acetylcholinesterase-Based Chronoamperometric Biosensor for Fast and Reliable Assay of Nerve Agents

Type

journal article in Web of Science

Language

English

Original Abstract

The enzyme acetylcholinesterase (AChE) is an important part of cholinergic nervous system, where it stops neurotransmission by hydrolysis of the neurotransmitter acetylcholine. It is sensitive to inhibition by organophosphate and carbamate insecticides, some Alzheimer disease drugs, secondary metabolites such as aflatoxins and nerve agents used in chemical warfare. When immobilized on a sensor (physico-chemical transducer), it can be used for assay of these inhibitors. In the experiments described herein, an AChE- based electrochemical biosensor using screen printed electrode systems was prepared. The biosensor was used for assay of nerve agents such as sarin, soman, tabun and VX. The limits of detection achieved in a measuring protocol lasting ten minutes were 7.41 x 10(-12) mol/L for sarin, 6.31 x 10(-12) mol/L for soman, 6.17 x 10(-11) mol/L for tabun, and 2.19 x 10(-11) mol/L for VX, respectively. The assay was reliable, with minor interferences caused by the organic solvents ethanol, methanol, isopropanol and acetonitrile. Isopropanol was chosen as suitable medium for processing lipophilic samples.

Keywords

biosensor; acetylcholinesterase; sarin; tabun; soman; VX; inhibitor; screen printed electrode; voltammetry; amperometry

Authors

POHANKA, M.; ADAM, V.; KIZEK, R.

Released

1. 9. 2013

Publisher

MDPI

ISBN

1424-8220

Periodical

SENSORS

Year of study

13

Number

9

State

Swiss Confederation

Pages from

11498

Pages to

11506

Pages count

7

URL

https://www.mdpi.com/1424-8220/13/9/11498

Full text in the Digital Library

http://hdl.handle.net/11012/137241

BibTex

@article{BUT143113,
  author="Miroslav {Pohanka} and Vojtěch {Adam} and René {Kizek}",
  title="An Acetylcholinesterase-Based Chronoamperometric Biosensor for Fast and Reliable Assay of Nerve Agents",
  journal="SENSORS",
  year="2013",
  volume="13",
  number="9",
  pages="11498--11506",
  doi="10.3390/s130911498",
  issn="1424-8220",
  url="https://www.mdpi.com/1424-8220/13/9/11498"
}