Publication detail

Accumulation of PHA granules protects bacterial cells from adverse effect of UV-irradiation

SLANINOVÁ, E. SEDLÁČEK, P. MRAVEC, F. HESKO, O. MÜLLEROVÁ, L. OBRUČA, S.

Original Title

Accumulation of PHA granules protects bacterial cells from adverse effect of UV-irradiation

English Title

Accumulation of PHA granules protects bacterial cells from adverse effect of UV-irradiation

Type

abstract

Language

en

Original Abstract

Bacteria developed various strategies to cope with stress conditions including UV irradiation. Polyhydroxyalkanoates (PHA) are polyesters accumulated in form of intracellular granules by numerous bacteria primarily as storage compounds, nevertheless, we investigated that PHA granules are not used only as carbon and energy storage but bacteria can use this compound also as UV-protectant. . Mechanisms of protective effect of PHA granules were determined by different spectroscopic approaches such as turbidity measurement, absorption spectrophotometry with integration sphere , nephelometry and continuous fluorescence microphotolysis. Generally, protective mechanisms of PHA granules is predominantly based on very effective scattering of UV radiation with wavelength of about 250 – 290 nm which protect photo-sensitive molecules, especially DNA, from their damage

English abstract

Bacteria developed various strategies to cope with stress conditions including UV irradiation. Polyhydroxyalkanoates (PHA) are polyesters accumulated in form of intracellular granules by numerous bacteria primarily as storage compounds, nevertheless, we investigated that PHA granules are not used only as carbon and energy storage but bacteria can use this compound also as UV-protectant. . Mechanisms of protective effect of PHA granules were determined by different spectroscopic approaches such as turbidity measurement, absorption spectrophotometry with integration sphere , nephelometry and continuous fluorescence microphotolysis. Generally, protective mechanisms of PHA granules is predominantly based on very effective scattering of UV radiation with wavelength of about 250 – 290 nm which protect photo-sensitive molecules, especially DNA, from their damage

Keywords

Cupravodius necator H16, UV protectant, nephelometry, turbidity

Released

11.09.2017

Location

Krakow

Pages from

166

Pages to

166

Pages count

1

BibTex


@misc{BUT142230,
  author="Eva {Slaninová} and Petr {Sedláček} and Filip {Mravec} and Ondrej {Hesko} and Lucie {Müllerová} and Stanislav {Obruča}",
  title="Accumulation of PHA granules protects bacterial cells from adverse effect of UV-irradiation",
  annote="Bacteria developed various strategies to cope with stress conditions including UV irradiation.  Polyhydroxyalkanoates (PHA) are polyesters accumulated in form of intracellular granules by numerous bacteria primarily as storage compounds, nevertheless, we investigated that PHA granules are not used only as carbon and energy storage  but bacteria can use this compound also as UV-protectant. . Mechanisms of protective effect of PHA granules were determined by different spectroscopic approaches such as   turbidity measurement, absorption spectrophotometry with integration sphere , nephelometry and continuous fluorescence microphotolysis. Generally, protective mechanisms of PHA granules is predominantly based on very effective scattering of UV radiation with wavelength of about 250 – 290 nm which protect photo-sensitive molecules, especially DNA, from their damage",
  booktitle="6th Central European Congress of life Sciences Eurobiotech",
  chapter="142230",
  howpublished="print",
  year="2017",
  month="september",
  pages="166--166",
  type="abstract"
}