Publication detail

Kinetic analysis of human metallothionein and CdTe quantum dot complexes using fluorescence and voltammetry techniques

GUSZPIT, E. KREJČOVÁ, L. KŘÍŽKOVÁ, S. KEPINSKA, M. RICHTERA, L. KOPEL, P. ADAM, V. MILNEROWITZ, H.

Original Title

Kinetic analysis of human metallothionein and CdTe quantum dot complexes using fluorescence and voltammetry techniques

Type

journal article in Web of Science

Language

English

Original Abstract

Thanks to quantum dots’ (QDs) properties, they can be used as selective and sensitive biomarkers in molecular imaging. In a previous paper, we confirmed the possibility of interaction between mercaptosuccinic acid-capped cadmium telluride QDs (MSA-CdTe) and human metallothionein (MT). The aim of this study was to expand on our previous research with an evaluation of the stability of the formed complexes between human MT and four CdTe compounds of the following sizes: 3.4 nm (blue QDs), 3.8 nm (green QDs), 4.5 nm (yellow QDs), and 5.2 nm (red QDs). Complexes were evaluated over time using fluorescence intensity and differential pulse voltammetry. Differences between the voltammograms obtained for standard solutions and for CdTe + MT show that complexes were formed. An increase in fluorescence intensity was observed for blue (Δ% ≈ 40 for t = 1 → 120 min) and red (Δ% ≈ 30 for t = 1 → 120 min) CdTe-MT complexes than CdTe alone, whereas green and yellow CdTe-MT complexes had a lower fluorescence intensity than CdTe alone. A stronger time dependence of the mercaptosuccinic acid (MSA) peak height on the timeline and differences in the MSA peak shape (in CdTe, and CdTe + MT complexes) were also observed by voltammetry. Authors noticed a decrease in the Cat2 signal of the red and green CdTe + MT complexes at the time of conjugation. Our results reveal that the size of QDs has an impact on the interaction between CdTe and human MT, as well as on the stability of complexes formed during these interactions. The bioconjugates’ stability was also found to depend on the time of interaction.

Keywords

Quantum dots; Metallothionein; Bioconjugation; Biomarkers; Electrochemistry; Differential pulse voltammetry

Authors

GUSZPIT, E.; KREJČOVÁ, L.; KŘÍŽKOVÁ, S.; KEPINSKA, M.; RICHTERA, L.; KOPEL, P.; ADAM, V.; MILNEROWITZ, H.

Released

1. 12. 2017

ISBN

0927-7765

Periodical

Colloids and Surfaces B: Biointerfaces

Year of study

160

Number

1

State

Kingdom of the Netherlands

Pages from

381

Pages to

389

Pages count

9

BibTex

@article{BUT140390,
  author="Ewelina {Guszpit} and Ludmila {Krejčová} and Soňa {Křížková} and Marta {Kepinska} and Lukáš {Richtera} and Pavel {Kopel} and Vojtěch {Adam} and Halina {Milnerowitz}",
  title="Kinetic analysis of human metallothionein and CdTe quantum dot complexes using fluorescence and voltammetry techniques",
  journal="Colloids and Surfaces B: Biointerfaces",
  year="2017",
  volume="160",
  number="1",
  pages="381--389",
  doi="10.1016/j.colsurfb.2017.09.033",
  issn="0927-7765"
}