Publication detail

Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy

JAROŠ, J. PETROV M. TESAŘOVÁ, M. HAMPL A.

Original Title

Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy

Type

book chapter

Language

English

Original Abstract

Cell culture methods have been developed in efforts to produce biologically relevant systems for developmental and disease modeling, and appropriate analytical tools are essential. Knowledge of ultrastructural characteristics represents the basis to reveal in situ the cellular morphology, cell-cell interactions, organelle distribution, niches in which cells reside, and many more. The traditional method for 3D visualization of ultrastructural components, serial sectioning using transmission electron microscopy (TEM), is very labor-intensive due to contentious TEM slice preparation and subsequent image processing of the whole collection. In this chapter, we present serial block-face scanning electron microscopy, together with complex methodology for spheroid formation, contrasting of cellular compartments, image processing, and 3D visualization. The described technique is effective for detailed morphological analysis of stem cell spheroids, organoids, as well as organotypic cell cultures.

Keywords

3D visualization, Image reconstruction, Image segmentation, Morphology, Organoid, SBF-SEM, Scanning electron microscopy, Serial block-face, Spheroid, Stem cell, Ultrastructure

Authors

JAROŠ, J.; PETROV M.; TESAŘOVÁ, M.; HAMPL A.

Released

21. 6. 2017

Publisher

Springer New York

Location

New York

ISBN

978-1-4939-7019-3

Book

3D Cell Culture: Methods and Protocols

Edition

1

Edition number

1612

Pages from

417

Pages to

431

Pages count

15

URL

https://link.springer.com/protocol/10.1007%2F978-1-4939-7021-6_30

BibTex

@inbook{BUT137420,
  author="JAROŠ, J. and PETROV M. and TESAŘOVÁ, M. and HAMPL A.",
  title="Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy",
  booktitle="3D Cell Culture: Methods and Protocols",
  year="2017",
  publisher="Springer New York",
  address="New York",
  series="1",
  edition="1612",
  pages="417--431",
  isbn="978-1-4939-7019-3",
  url="https://link.springer.com/protocol/10.1007%2F978-1-4939-7021-6_30"
}