Publication detail

Hydrophobic nanodomains in hyaluronan-surfactant system: a fluorescence anisotropy study

HOLÍNKOVÁ, P. MRAVEC, F. PEKAŘ, M.

Original Title

Hydrophobic nanodomains in hyaluronan-surfactant system: a fluorescence anisotropy study

English Title

Hydrophobic nanodomains in hyaluronan-surfactant system: a fluorescence anisotropy study

Type

conference paper

Language

en

Original Abstract

This paper deals with the fluorescence anisotropy study of hydrophobic nanodomains formed by interaction between hyaluronan (Hya) and cationic surfactant cetyltrimethylammonium bromide (CTAB) below critical micelle concentration in aqueous solution and the effect of sodium chloride addition to this system. Hyaluronan as negatively charged polyelectrolyte can interact with cationic surfactants via electrostatic interactions to form aggregates, which could be potential used for targeted drug delivery. Two fluorescent probes, which are solubilized in hydrophobic region, were used – perylene and diphenylhexatrien (DPH). The emission spectra, total intensity of fluorescence and steady-state anisotropy of fluorescence in the samples were measured. It was observed that initial addition of CTAB to hyaluronan aqueous solution leads to forming some small hydrophobic domains linked to hyaluronan chains. Then an increasing concentration of CTAB causes phase separation and formation of a condensed phase. Addition of sodium chloride to the samples leads to reorganization of this system – the condensed phase is dissolved and probably free micelles (possibly mixture of micelle linked to hyaluronan chain with free micelle) are formed in the solution.

English abstract

This paper deals with the fluorescence anisotropy study of hydrophobic nanodomains formed by interaction between hyaluronan (Hya) and cationic surfactant cetyltrimethylammonium bromide (CTAB) below critical micelle concentration in aqueous solution and the effect of sodium chloride addition to this system. Hyaluronan as negatively charged polyelectrolyte can interact with cationic surfactants via electrostatic interactions to form aggregates, which could be potential used for targeted drug delivery. Two fluorescent probes, which are solubilized in hydrophobic region, were used – perylene and diphenylhexatrien (DPH). The emission spectra, total intensity of fluorescence and steady-state anisotropy of fluorescence in the samples were measured. It was observed that initial addition of CTAB to hyaluronan aqueous solution leads to forming some small hydrophobic domains linked to hyaluronan chains. Then an increasing concentration of CTAB causes phase separation and formation of a condensed phase. Addition of sodium chloride to the samples leads to reorganization of this system – the condensed phase is dissolved and probably free micelles (possibly mixture of micelle linked to hyaluronan chain with free micelle) are formed in the solution.

Keywords

hyaluronan, surfactants, fluorescence spectroscopy, anisotropy

Released

19.10.2016

Publisher

TANGER Ltd.

Location

Ostrava

ISBN

978-80-87294-71-0

Book

NANOCON 2016 Conference Proceedings

Edition

1st edition, 2017

Pages from

444

Pages to

448

Pages count

5

BibTex


@inproceedings{BUT137110,
  author="Petra {Holínková} and Filip {Mravec} and Miloslav {Pekař}",
  title="Hydrophobic nanodomains in hyaluronan-surfactant system: a fluorescence anisotropy study
",
  annote="This paper deals with the fluorescence anisotropy study of hydrophobic nanodomains formed by interaction between hyaluronan (Hya) and cationic surfactant cetyltrimethylammonium bromide (CTAB) below critical micelle concentration in aqueous solution and the effect of sodium chloride addition to this system. Hyaluronan as negatively charged polyelectrolyte can interact with cationic surfactants via electrostatic interactions to form aggregates, which could be potential used for targeted drug delivery. Two fluorescent probes, which are solubilized in hydrophobic region, were used – perylene and diphenylhexatrien (DPH). The emission spectra, total intensity of fluorescence and steady-state anisotropy of fluorescence in the samples were measured. It was observed that initial addition of CTAB to hyaluronan aqueous solution leads to forming some small hydrophobic domains linked to hyaluronan chains. Then an increasing concentration of CTAB causes phase separation and formation of a condensed phase. Addition of sodium chloride to the samples leads to reorganization of this system – the condensed phase is dissolved and probably free micelles (possibly mixture of micelle linked to hyaluronan chain with free micelle) are formed in the solution.",
  address="TANGER Ltd.",
  booktitle="NANOCON 2016 Conference Proceedings",
  chapter="137110",
  edition="1st edition, 2017",
  howpublished="electronic, physical medium",
  institution="TANGER Ltd.",
  year="2016",
  month="october",
  pages="444--448",
  publisher="TANGER Ltd.",
  type="conference paper"
}