Publication detail

The Application of Curve Fitting on the Voltammograms of Various Isoforms of Metallothioneins–Metal Complexes

MERLOS RODRIGO, M. LÓPEZ MOLINA, J. JIMENEZ JIMENEZ, A. PLANELLS DEL POZO, E. ADAM, P. ECKSCHLAGER, T. ZÍTKA, O. RICHTERA, L. ADAM, V.

Original Title

The Application of Curve Fitting on the Voltammograms of Various Isoforms of Metallothioneins–Metal Complexes

Type

journal article in Web of Science

Language

English

Original Abstract

The translation of metallothioneins (MTs) is one of the defense strategies by which organisms protect themselves from metal-induced toxicity. MTs belong to a family of proteins comprising MT-1, MT-2, MT-3, and MT-4 classes, with multiple isoforms within each class. The main aim of this study was to determine the behavior of MT in dependence on various externally modelled environments, using electrochemistry. In our study, the mass distribution of MTs was characterized using MALDI-TOF. After that, adsorptive transfer stripping technique with differential pulse voltammetry was selected for optimization of electrochemical detection of MTs with regard to accumulation time and pH effects. Our results show that utilization of 0.5 M NaCl, pH 6.4, as the supporting electrolyte provides a highly complicated fingerprint, showing a number of non-resolved voltammograms. Hence, we further resolved the voltammograms exhibiting the broad and overlapping signals using curve fitting. The separated signals were assigned to the electrochemical responses of several MT complexes with zinc(II), cadmium(II), and copper(II), respectively. Our results show that electrochemistry could serve as a great tool for metalloproteomic applications to determine the ratio of metal ion bonds within the target protein structure, however, it provides highly complicated signals, which require further resolution using a proper statistical method, such as curve fitting.

Keywords

electrochemistry; mass spectrometry MALDI-TOF; metallothionein; metallomics; signal resolving

Authors

MERLOS RODRIGO, M.; LÓPEZ MOLINA, J.; JIMENEZ JIMENEZ, A.; PLANELLS DEL POZO, E.; ADAM, P.; ECKSCHLAGER, T.; ZÍTKA, O.; RICHTERA, L.; ADAM, V.

Released

1. 3. 2017

Publisher

MDPI

ISBN

1422-0067

Periodical

International Journal of Molecular Sciences

Year of study

18

Number

3

State

Swiss Confederation

Pages from

1

Pages to

11

Pages count

11

URL

Full text in the Digital Library

BibTex

@article{BUT135984,
  author="MERLOS RODRIGO, M. and LÓPEZ MOLINA, J. and JIMENEZ JIMENEZ, A. and PLANELLS DEL POZO, E. and ADAM, P. and ECKSCHLAGER, T. and ZÍTKA, O. and RICHTERA, L. and ADAM, V.",
  title="The Application of Curve Fitting on the Voltammograms of Various Isoforms of Metallothioneins–Metal Complexes",
  journal="International Journal of Molecular Sciences",
  year="2017",
  volume="18",
  number="3",
  pages="1--11",
  doi="10.3390/ijms18030610",
  issn="1422-0067",
  url="http://www.mdpi.com/1422-0067/18/3/610"
}