Publication detail

Flotate receptor-targeted liposomes with encapsulated anti-sarcosine antibodies for attenuation of prostate tumors

DOSTÁLOVÁ, S. HEGER, Z. POLANSKÁ, H. MERLOS RODRIGO, M. GURÁŇ, R. KULICH, P. KOPEL, P. MASAŘÍK, M. ECKSCHLAGER, T. STIBOROVÁ, M. KIZEK, R. ADAM, V.

Original Title

Flotate receptor-targeted liposomes with encapsulated anti-sarcosine antibodies for attenuation of prostate tumors

Type

abstract

Language

English

Original Abstract

Prostate cancer is the most occurring malignancy in men from USA or Western Europe with 29% of men of 30 to 40 years of age and 64% of men of 60 to 70 years of age suffering from prostate cancer [1]. One of the delineated oncometabolites in prostate cancer is the amino acid sarcosine (N-methylglycine) that plays a substantial role in progression of prostate cancer[2]. In this work, anti-sarcosine antibodies were employed for attenuation of prostate tumours in xenograft mice. The antibodies were modified with CdTe quantum dots for the detection of their intracellular delivery. Free antibodies are unable to enter intracellular space, so to enable internalization of these antibodies in the tumour intracellular space needed for subsequent neutralization of sarcosine, the antibodies were encapsulated in liposomes with loading efficiency of 69.2%. To increase the shuttling efficiency of antibodies-loaded liposomes, their surface was modified with folate, enabling entering of tumour cells via clathrin-mediated endocytosis[ 3], due to significant overexpression of folate receptors in a wide variety of cancer cells, including prostate cancer[4]. The resulting particles showed an average particle size of 172 nm and zeta potential of +22 mV at pH 7.4 (Fig. 1A). The successful modification with folic acid was confirmed using absorbance (Fig. 1B) and fluorescence (Fig. 1C) spectra of the nanoparticles.

Keywords

receptor-targeted liposomes; encapsulated; antibodies; nanoparticles; prostate cancer

Authors

DOSTÁLOVÁ, S.; HEGER, Z.; POLANSKÁ, H.; MERLOS RODRIGO, M.; GURÁŇ, R.; KULICH, P.; KOPEL, P.; MASAŘÍK, M.; ECKSCHLAGER, T.; STIBOROVÁ, M.; KIZEK, R.; ADAM, V.

Released

26. 6. 2016

ISBN

2296-9667

Periodical

European & Global Summit for Clinical Nanomedicine and Targeted Medicine

Year of study

2016

Number

9

State

Swiss Confederation

Pages from

201

Pages to

202

Pages count

2

BibTex

@misc{BUT132082,
  author="DOSTÁLOVÁ, S. and HEGER, Z. and POLANSKÁ, H. and MERLOS RODRIGO, M. and GURÁŇ, R. and KULICH, P. and KOPEL, P. and MASAŘÍK, M. and ECKSCHLAGER, T. and STIBOROVÁ, M. and KIZEK, R. and ADAM, V.",
  title="Flotate receptor-targeted liposomes with encapsulated anti-sarcosine antibodies for attenuation of prostate tumors",
  booktitle="European and Global Summit for Clinical Nanomedicine and Targeted Medicine",
  year="2016",
  journal="European & Global Summit for Clinical Nanomedicine and Targeted Medicine",
  volume="2016",
  number="9",
  pages="201--202",
  issn="2296-9667",
  note="abstract"
}