Publication detail

Combination of X-ray micro-Computed tomography and laser-ablation based analytical techniques for 3D high resolution elemental mapping

TESAŘOVÁ, M. POŘÍZKA, P. ŠEJNOHOVÁ, M. ZIKMUND, T. NOVOTNÝ, K. SANCEY, L. MOTTO-ROS, V. TILLEMENT, O. KAISER, J.

Original Title

Combination of X-ray micro-Computed tomography and laser-ablation based analytical techniques for 3D high resolution elemental mapping

Type

conference paper

Language

English

Original Abstract

Detection and chemical 3D mapping of nanoparticles (NPs) distribution in biospecimen is a challenging task that can be met by simultaneous utilization of imaging techniques. Here we focus on the synergy of X-Ray micro-Computed Tomography (μCT), Laser-Induced Breakdown Spectroscopy (LIBS) and Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) for investigation of various nanoparticles (Gd, Ti, Pb, etc.) distributed in organs. Special emphasis is given to the analysis of nanoparticles in a murine kidney in 3D. Firstly, we have demonstrated the 3D visualization capabilities in soft tissues by iodine staining with μCT. Next, subsequent LIBS and LA-ICP-MS analysis enabled to identify nanoparticles distribution in the sample. Thus, this combination provides a novel analytical approach how to quickly visualize and recognize different elements in soft tissues.

Keywords

Nanoparticles; 3D mapping; Computed Tomography; Laser-Induced Breakdown Spectroscopy; Laser Ablation Inductively Coupled Plasma Mass Spectrometry

Authors

TESAŘOVÁ, M.; POŘÍZKA, P.; ŠEJNOHOVÁ, M.; ZIKMUND, T.; NOVOTNÝ, K.; SANCEY, L.; MOTTO-ROS, V.; TILLEMENT, O.; KAISER, J.

Released

19. 10. 2016

ISBN

978-80-904959-4-4

Book

13th International Interdisciplinary Meeting on Bioanalysis

Edition number

13

Pages from

253

Pages to

255

Pages count

3

BibTex

@inproceedings{BUT130420,
  author="Markéta {Kaiser} and Pavel {Pořízka} and Marie {Šejnohová} and Tomáš {Zikmund} and Karel {Novotný} and Lucie {Sancey} and Vincent {Motto-Ros} and Olivier {Tillement} and Jozef {Kaiser}",
  title="Combination of X-ray micro-Computed tomography and laser-ablation based analytical techniques for 3D high resolution elemental mapping",
  booktitle="13th International Interdisciplinary Meeting on Bioanalysis",
  year="2016",
  number="13",
  pages="253--255",
  isbn="978-80-904959-4-4"
}