Publication detail

Effect of Hyaluronan on Diffusion Properties of Artificial Cell Membrane Studied by Fluorescence Correlation Spectroscopy

ADAMCOVÁ, Z. MRAVEC, F. PEKAŘ, M.

Original Title

Effect of Hyaluronan on Diffusion Properties of Artificial Cell Membrane Studied by Fluorescence Correlation Spectroscopy

English Title

Effect of Hyaluronan on Diffusion Properties of Artificial Cell Membrane Studied by Fluorescence Correlation Spectroscopy

Type

conference paper

Language

en

Original Abstract

Supported lipid bilayers were used as simplified models of cell membranes to study the effect of hyaluronan solution on their diffusion properties. The bilayers were prepared from lecithin liposomes in phosphate buffer using the vesicle fusion method. Hydrophilized cover glass slides were chosen as the supporting substrate. Two fluorescence probes – Nile Red and DiO were utilized to quantify the diffusion properties of the bilayer with and without presence of hyaluronan. For this purpose Z-scan Fluorescence Correlation Spectroscopy, which is a method developed specially for planar samples, was used. It was found out, that presence of this biopolymer causes slowing of the fluorescence probe diffusion within the bilayer.

English abstract

Supported lipid bilayers were used as simplified models of cell membranes to study the effect of hyaluronan solution on their diffusion properties. The bilayers were prepared from lecithin liposomes in phosphate buffer using the vesicle fusion method. Hydrophilized cover glass slides were chosen as the supporting substrate. Two fluorescence probes – Nile Red and DiO were utilized to quantify the diffusion properties of the bilayer with and without presence of hyaluronan. For this purpose Z-scan Fluorescence Correlation Spectroscopy, which is a method developed specially for planar samples, was used. It was found out, that presence of this biopolymer causes slowing of the fluorescence probe diffusion within the bilayer.

Keywords

Supported lipid bilayers, diffusion, Fluorescence Correlation Spectroscopy, Nile Red, DiO, Hyaluronan

Released

06.06.2016

Publisher

Masaryk University, Brno

Location

Brno

ISBN

978-80-210-8267-0

Book

XVI. Workshop of Physical Chemists and Electrochemists

Pages from

135

Pages to

138

Pages count

4

BibTex


@inproceedings{BUT128980,
  author="Zuzana {Adamcová} and Filip {Mravec} and Miloslav {Pekař}",
  title="Effect of Hyaluronan on Diffusion Properties of Artificial Cell Membrane Studied by Fluorescence Correlation Spectroscopy",
  annote="Supported lipid bilayers were used as simplified models of cell membranes to study the effect of hyaluronan solution on their diffusion properties. The bilayers were prepared from lecithin liposomes in phosphate buffer using the vesicle fusion method. Hydrophilized cover glass slides were chosen as the supporting substrate. Two fluorescence probes – Nile Red and DiO were utilized to quantify the diffusion properties of the bilayer with and without presence of hyaluronan. For this purpose Z-scan Fluorescence Correlation Spectroscopy, which is a method developed specially for planar samples, was used. It was found out, that presence of this biopolymer causes slowing of the fluorescence probe diffusion within the bilayer.",
  address="Masaryk University, Brno",
  booktitle="XVI. Workshop of Physical Chemists and Electrochemists",
  chapter="128980",
  howpublished="electronic, physical medium",
  institution="Masaryk University, Brno",
  year="2016",
  month="june",
  pages="135--138",
  publisher="Masaryk University, Brno",
  type="conference paper"
}