Publication detail
MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery
SVOBODA, O. FOHLEROVÁ, Z. BAIAZITOVA, L. ČMIEL, V. HUBÁLEK, J. PROVAZNÍK, I. PEKAŘÍK, V.
Original Title
MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery
English Title
MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery
Type
conference paper
Language
en
Original Abstract
The non-toxic intracellular delivery of biopolymers is very hot topic in current biology. In this work, two different MATRA-A reagent/polyethyleneimine transfection protocols were used for intracellular delivery of ChR2 DNA vector. Cell morphology changes were studied 48 hours after transfection. Our transfection efficiency is lower than normally achieved, but the rate of cells with physiological properties remains is very high: 85.34%. Therefore, these transfection protocols are very applicable for electrophysiological experiments.
English abstract
The non-toxic intracellular delivery of biopolymers is very hot topic in current biology. In this work, two different MATRA-A reagent/polyethyleneimine transfection protocols were used for intracellular delivery of ChR2 DNA vector. Cell morphology changes were studied 48 hours after transfection. Our transfection efficiency is lower than normally achieved, but the rate of cells with physiological properties remains is very high: 85.34%. Therefore, these transfection protocols are very applicable for electrophysiological experiments.
Keywords
transfection, MATRA-A reagent, plasmid delivery
Released
06.05.2016
Publisher
Masarykova Univerzita - MUNI PRESS
Location
Brno, Czech Republic
ISBN
978-80-210-8267-0
Book
XVI. Workshop of Physical Chemists and Electrochemists
Edition
1
Pages from
1
Pages to
3
Pages count
3
BibTex
@inproceedings{BUT128268,
author="Ondřej {Svoboda} and Zdenka {Fohlerová} and Larisa {Baiazitova} and Vratislav {Čmiel} and Jaromír {Hubálek} and Ivo {Provazník} and Vladimír {Pekařík}",
title="MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery",
annote="The non-toxic intracellular delivery of biopolymers is very hot topic in current biology. In this work, two different MATRA-A reagent/polyethyleneimine transfection protocols were used for intracellular delivery of ChR2 DNA vector. Cell morphology changes were studied 48 hours after transfection. Our transfection efficiency is lower than normally achieved, but the rate of cells with physiological properties remains is very high: 85.34%. Therefore, these transfection protocols are very applicable for electrophysiological experiments.",
address="Masarykova Univerzita - MUNI PRESS",
booktitle="XVI. Workshop of Physical Chemists and Electrochemists",
chapter="128268",
edition="1",
howpublished="electronic, physical medium",
institution="Masarykova Univerzita - MUNI PRESS",
year="2016",
month="may",
pages="1--3",
publisher="Masarykova Univerzita - MUNI PRESS",
type="conference paper"
}