Publication detail

3D printed stratospheric probe as a platform for determination of DNA damage based on carbon quantum dots/DNA complex fluorescence increase

HEGER, Z. ZÍTKA, J. NEJDL, L. MOULICK, A. MILOSAVLJEVIĆ, V. KOPEL, P. ZÁVODSKÝ, O. KAPUŠ, J. LENŽA, L. ŘEZKA, M. ADAM, V. KIZEK, R.

Original Title

3D printed stratospheric probe as a platform for determination of DNA damage based on carbon quantum dots/DNA complex fluorescence increase

Type

journal article in Web of Science

Language

English

Original Abstract

We present a utilization of carbon quantum dots (CQDs), passivated with polyethylene glycol as a fluorescent recognition probe for DNA damage. Synthesized CQDs were characterized in detail, using optical and electrochemical methods. Further, fluorescent behavior of CQDs was monitored in the presence of genomic DNA, isolated from Staphylococcus aureus. In laboratory conditions, after 30 min of exposure to UV irradiation (k = 254 nm), the DNA/CQDs complex significantly increased its fluorescence. Further, stratospheric probe was suggested and crafted by using technology of 3D printing (acrylonitrile–butadiene–styrene as a material). CQDs were exploited to evaluate the DNA damage in stratospheric conditions (up to 20,000 m) by determination of fluorescence increase (kexc = 245 nm, kem = 400 nm), together with other parameters (temperature, humidity, altitude, pressure, UV intensity, and X-ray irradiation). The obtained data showed that the sensor utilizing the DNA/ CQDs was able to identify the DNA damage, together with external conditions. It was shown the proposed concept is able to operate at temperatures lower than -70 C. The proposed protocol may by applicable as a biosensor for long-term space missions, like international space station, missions to the Moon or Mars.

Keywords

Carbon nanomaterials, Near-space conditions, Radiation, Ultraviolet spectrum

Authors

HEGER, Z.; ZÍTKA, J.; NEJDL, L.; MOULICK, A.; MILOSAVLJEVIĆ, V.; KOPEL, P.; ZÁVODSKÝ, O.; KAPUŠ, J.; LENŽA, L.; ŘEZKA, M.; ADAM, V.; KIZEK, R.

Released

1. 5. 2016

ISBN

1434-4475

Periodical

Monatshefte für Chemie

Year of study

147

Number

5

State

Republic of Austria

Pages from

873

Pages to

880

Pages count

8

BibTex

@article{BUT127766,
  author="Zbyněk {Heger} and Jan {Zítka} and Lukáš {Nejdl} and Amitava {Moulick} and Vedran {Milosavljević} and Pavel {Kopel} and Ondrej {Závodský} and Jakub {Kapuš} and Libor {Lenža} and Milan {Řezka} and Vojtěch {Adam} and René {Kizek}",
  title="3D printed stratospheric probe as a platform for determination of DNA damage based on carbon quantum dots/DNA complex fluorescence increase",
  journal="Monatshefte für Chemie",
  year="2016",
  volume="147",
  number="5",
  pages="873--880",
  doi="10.1007/s00706-016-1705-y",
  issn="1434-4475"
}