Publication detail

Analysis of neural crest-derived clones reveals novel aspects of facial development

KAUCKÁ, M. IVASHKIN E. GYLBORG, D. ZIKMUND, T. TESAŘOVÁ, M. KAISER, J. XIE, M. PETERSEN, J. PACHNIS, V. NICLOIS, S.K. YU, T. SHARPE, P. ARENAS, E. BRISMAR, H. BLOM, H. CLEVERS, H. SUTER, U. CHAGIN, A.S. FRIED, K. HELLANDER, A. ADAMEYKO, I.

Original Title

Analysis of neural crest-derived clones reveals novel aspects of facial development

Type

journal article in Web of Science

Language

English

Original Abstract

Cranial neural crest cells populate the future facial region and produce ectomesenchyme-derived tissues, such as cartilage, bone, dermis, smooth muscle, adipocytes, and many others. However, the contribution of individual neural crest cells to certain facial locations and the general spatial clonal organization of the ectomesenchyme have not been determined. We investigated how neural crest cells give rise to clonally organized ectomesenchyme and how this early ectomesenchyme behaves during the developmental processes that shape the face. Using a combination of mouse and zebrafish models, we analyzed individual migration, cell crowd movement, oriented cell division, clonal spatial overlapping, and multilineage differentiation. The early face appears to be built from multiple spatially defined overlapping ectomesenchymal clones. During early face development, these clones remain oligopotent and generate various tissues in a given location. By combining clonal analysis, computer simulations, mouse mutants, and live imaging, we show that facial shaping results from an array of local cellular activities in the ectomesenchyme. These activities mostly involve oriented divisions and crowd movements of cells during morphogenetic events. Cellular behavior that can be recognized as individual cell migration is very limited and short-ranged and likely results from cellular mixing due to the proliferation activity of the tissue. These cellular mechanisms resemble the strategy behind limb bud morphogenesis, suggesting the possibility of common principles and deep homology between facial and limb outgrowth.

Keywords

facial development, analysis, neural crest cells

Authors

KAUCKÁ, M.; IVASHKIN E.; GYLBORG, D.; ZIKMUND, T.; TESAŘOVÁ, M.; KAISER, J.; XIE, M.; PETERSEN, J.; PACHNIS, V.; NICLOIS, S.K.; YU, T.; SHARPE, P.; ARENAS, E.; BRISMAR, H.; BLOM, H.; CLEVERS, H.; SUTER, U.; CHAGIN, A.S.; FRIED, K.; HELLANDER, A.; ADAMEYKO, I.

Released

3. 8. 2016

Publisher

American Association for the Advancement of Science (AAAS)

Location

Washington, DC, USA

ISBN

2375-2548

Periodical

Science Advances

Year of study

2

Number

8

State

United States of America

Pages from

1

Pages to

16

Pages count

15

URL

http://advances.sciencemag.org/content/2/8/e1600060

Full text in the Digital Library

http://hdl.handle.net/11012/63069

BibTex

@article{BUT127300,
  author="KAUCKÁ, M. and IVASHKIN E. and GYLBORG, D. and ZIKMUND, T. and TESAŘOVÁ, M. and KAISER, J. and XIE, M. and PETERSEN, J. and PACHNIS, V. and NICLOIS, S.K. and YU, T. and SHARPE, P. and ARENAS, E. and BRISMAR, H. and BLOM, H. and CLEVERS, H. and SUTER, U. and CHAGIN, A.S. and FRIED, K. and HELLANDER, A. and ADAMEYKO, I.",
  title="Analysis of neural crest-derived clones reveals novel aspects of facial development",
  journal="Science Advances",
  year="2016",
  volume="2",
  number="8",
  pages="1--16",
  doi="10.1126/sciadv.1600060",
  issn="2375-2548",
  url="http://advances.sciencemag.org/content/2/8/e1600060"
}