Publication detail

Steady-State Fluorescence Spectroscopy Techniques in Determination of Phospholipids Vesicles Properties

BURDÍKOVÁ, J. MRAVEC, F. PEKAŘ, M.

Original Title

Steady-State Fluorescence Spectroscopy Techniques in Determination of Phospholipids Vesicles Properties

English Title

Steady-State Fluorescence Spectroscopy Techniques in Determination of Phospholipids Vesicles Properties

Type

abstract

Language

en

Original Abstract

Steady-state fluorescence techniques represent a powerful tool for the determination of great variety of phospholipids vesicles (liposome) properties. Phase transition temperature is one of the most important characteristics of liposomes. It is commonly determined by means of calorimetric technics. It can be also obtained by analyzing of laurdan emission spectra at different temperatures. The degree of a lipid acyl chain order in liposomes and a membrane fluidity can by studied by means of fluorescence anisotropy, which measures depolarization of fluorophores excited by polarized light, where the depolarization depends on the membrane fluidity. Diphenylhexatriene is a suitable fluorescence probe for the anisotropy measurements. The aggregation behavior of lipid molecules can be studied using fluorescence probes such as pyrene or perylene. These fluorescence probes are sensitive to the polarity of the local environment and indicate changes in lipid aggregates character. Fluorescent lipophilic tracers such a DiO or DiA are usually applied in a staining of the cell surface, but they can be easily used also in aggregates formation study.

English abstract

Steady-state fluorescence techniques represent a powerful tool for the determination of great variety of phospholipids vesicles (liposome) properties. Phase transition temperature is one of the most important characteristics of liposomes. It is commonly determined by means of calorimetric technics. It can be also obtained by analyzing of laurdan emission spectra at different temperatures. The degree of a lipid acyl chain order in liposomes and a membrane fluidity can by studied by means of fluorescence anisotropy, which measures depolarization of fluorophores excited by polarized light, where the depolarization depends on the membrane fluidity. Diphenylhexatriene is a suitable fluorescence probe for the anisotropy measurements. The aggregation behavior of lipid molecules can be studied using fluorescence probes such as pyrene or perylene. These fluorescence probes are sensitive to the polarity of the local environment and indicate changes in lipid aggregates character. Fluorescent lipophilic tracers such a DiO or DiA are usually applied in a staining of the cell surface, but they can be easily used also in aggregates formation study.

Keywords

steady-state fluorescence spectroscopy, lipid, liposome, pyrene, laurdan, fluorescent lipophilic tracers, fluorescence anisotropy, DPH

Released

03.12.2015

Publisher

Vysoké učení technické v Brně, Fakulta chemická

Location

Brno

ISBN

978-80-214-5290-9

Book

Sborník abstraktů: Studentská konference Chemie je život

Edition number

první

Pages from

61

Pages to

61

Pages count

1

BibTex


@misc{BUT124991,
  author="Jana {Burdíková} and Filip {Mravec} and Miloslav {Pekař}",
  title="Steady-State Fluorescence Spectroscopy Techniques in Determination of Phospholipids Vesicles Properties",
  annote="Steady-state fluorescence techniques represent a powerful tool for the determination of great variety of phospholipids vesicles (liposome) properties. Phase transition temperature is one of the most important characteristics of liposomes. It is commonly determined by means of calorimetric technics. It can be also obtained by analyzing of laurdan emission spectra at different temperatures.
The degree of a lipid acyl chain order in liposomes and a membrane fluidity can by studied by means of fluorescence anisotropy, which measures depolarization of fluorophores excited by polarized light, where the depolarization depends on the membrane fluidity. Diphenylhexatriene is a suitable fluorescence probe for the anisotropy measurements.
The aggregation behavior of lipid molecules can be studied using fluorescence probes such as pyrene or perylene. These fluorescence probes are sensitive to the polarity of the local environment and indicate changes in lipid aggregates character. Fluorescent lipophilic tracers such a DiO or DiA are usually applied in a staining of the cell surface, but they can be easily used also in aggregates formation study.",
  address="Vysoké učení technické v Brně, Fakulta chemická",
  booktitle="Sborník abstraktů: Studentská konference Chemie je život",
  chapter="124991",
  howpublished="print",
  institution="Vysoké učení technické v Brně, Fakulta chemická",
  year="2015",
  month="december",
  pages="61--61",
  publisher="Vysoké učení technické v Brně, Fakulta chemická",
  type="abstract"
}