Publication detail

Label-free and amplification-free miR-124 detection in human cells

ŠMERKOVÁ, K. HUDCOVÁ, K. VLAHOVÁ, V. VACULOVIČOVÁ, M. PEKAŘÍK, V. MASAŘÍK, M. ADAM, V. KÍZEK, R.

Original Title

Label-free and amplification-free miR-124 detection in human cells

Type

journal article in Web of Science

Language

English

Original Abstract

MicroRNAs (miRNAs) are becoming a very important group of molecules especially since their connection to numerous diseases has been revealed. The potential in gene therapy as well as in diagnostics is being widely investigated leading to the demand of sensitive, selective and simple methods of isolation and detection. The combined advantages of magnetic particle-based separation with sensitive electrochemical detection may offer a very valuable tool for these purposes. In this study, the miR-124 was targeted as an example analyte for development and optimization of the isolation procedure coupled to the electrochemical detection. The sensitivity of the method was demonstrated by the limit of detection at the level of nanomolar concentration (4 nM). To verify the applicability of the procedure to the real samples, miR-124 was isolated from the human embryonic kidney cells naturally expressing this miRNA molecule and the results were compared to the amount of miR-124 isolated from the cells transfected by the pENTR-miR-124 plasmid leading to the overexpreskon of miR-124.

Keywords

miRNA; prostate cancer; magnetic particles; isolation

Authors

ŠMERKOVÁ, K.; HUDCOVÁ, K.; VLAHOVÁ, V.; VACULOVIČOVÁ, M.; PEKAŘÍK, V.; MASAŘÍK, M.; ADAM, V.; KÍZEK, R.

RIV year

2015

Released

1. 1. 2015

ISBN

1019-6439

Periodical

INTERNATIONAL JOURNAL OF ONCOLOGY

Year of study

46

Number

2

State

Hellenic Republic

Pages from

871

Pages to

877

Pages count

7

BibTex

@article{BUT123360,
  author="Kristýna {Šmerková} and Kristýna {Hudcová} and Veronika {Vlahová} and Markéta {Vaculovičová} and Vladimír {Pekařík} and Michal {Masařík} and Vojtěch {Adam} and René {Kizek}",
  title="Label-free and amplification-free miR-124 detection in human cells",
  journal="INTERNATIONAL JOURNAL OF ONCOLOGY",
  year="2015",
  volume="46",
  number="2",
  pages="871--877",
  doi="10.3892/ijo.2014.2756",
  issn="1019-6439"
}