Publication detail

Label-free bead-based metallothionein electrochemical immunosensor

NEJDL, L. NGUYEN, H. RICHTERA, L. KŘÍŽKOVÁ, S. GURÁŇ, R. MASAŘÍK, M. HYNEK, D. HEGER, Z. LUNDBERG, K. ERIKSON, K. ADAM, V. KÍZEK, R.

Original Title

Label-free bead-based metallothionein electrochemical immunosensor

Type

journal article in Web of Science

Language

English

Original Abstract

A novel microfluidic label-free bead-based metallothionein immunosensors was designed. To the surface of superparamagnetic agarose beads coated with protein A, polyclonal chicken IgY specifically recognizing metallothionein (MT) were immobilized via rabbit IgG. The Brdicka reaction was used for metallothionein detection in a microfluidic printed 3D chip. The assembled chip consisted of a single copper wire coated with a thin layer of amalgam as working electrode. Optimization of MT detection using designed microfluidic chip was performed in stationary system as well as in the flow arrangement at various flow rates (0-1800 L/min). In stationary arrangement it is possible to detect MT concentrations up to 30ng/mL level, flow arrangement allows reliable detection of even lower concentration (12.5ng/mL). The assembled miniature flow chip was subsequently tested for the detection of MT elevated levels (at approx. level 100 g/mL) in samples of patients with cancer. The stability of constructed device for metallothionein detection in flow arrangement was found to be several days without any maintenance needed.

Keywords

Immunosensor; Label-free; Metallothionein; Superparamagnetic particles

Authors

NEJDL, L.; NGUYEN, H.; RICHTERA, L.; KŘÍŽKOVÁ, S.; GURÁŇ, R.; MASAŘÍK, M.; HYNEK, D.; HEGER, Z.; LUNDBERG, K.; ERIKSON, K.; ADAM, V.; KÍZEK, R.

RIV year

2015

Released

1. 4. 2015

ISBN

0173-0835

Periodical

Electrophoresis

Year of study

36

Number

16

State

Federal Republic of Germany

Pages from

1894

Pages to

1904

Pages count

11

BibTex

@article{BUT123339,
  author="Lukáš {Nejdl} and Kristofer {Erikson} and Vojtěch {Adam} and Hoai Viet {Nguyen} and Lukáš {Richtera} and Soňa {Křížková} and Roman {Guráň} and Michal {Masařík} and David {Hynek} and Zbyněk {Heger} and Karin {Lundberg} and René {Kizek}",
  title="Label-free bead-based metallothionein electrochemical immunosensor",
  journal="Electrophoresis",
  year="2015",
  volume="36",
  number="16",
  pages="1894--1904",
  doi="10.1002/elps.201500069",
  issn="0173-0835"
}