Publication detail

Papillomavirus infection of roe deer in the Czech Republic and fibropapilloma-associated levels of metallothionein, zinc, and oxidative stress

KRÁL, J. BANĎOUCHOVÁ, H. BRICHTA, J. KOVACOVA, V. ONDRÁČEK, K. OSIČKOVÁ, J. HRUBÁ, H. HUTAŘOVÁ, Z. KOMÍNKOVÁ, M. CERNEI, N. KONEČNÁ, M. TMEJOVÁ, K. ZÍTKA, O. ADAM, V. KÍZEK, R. SKORIC, M. TREMLOVÁ, B. TREML, F. PIKULA, J.

Original Title

Papillomavirus infection of roe deer in the Czech Republic and fibropapilloma-associated levels of metallothionein, zinc, and oxidative stress

Type

journal article in Web of Science

Language

English

Original Abstract

The present study aimed at the aetiological diagnosis of skin tumours of roe deer (Capreolus capreolus) in the Czech Republic. A total of 33 roe deer specimens showing skin masses were sampled for histopathology, virus detection and identification using polymerase chain reaction (PCR) and deoxyribonucleic acid sequence (DNA) analysis, and to investigate fibropapilloma-associated levels of metallothionein, zinc and oxidative stress in 2012 and 2013. Ticks (Ixodes ricinus) and deer keds (Lipoptena cervi) were also collected and pooled from sampled game specimens. Ticks found on dogs in hunting grounds under study were pooled into another sample. Skin tumours, ranging from 2 to 10 per inspected specimen and 2 to 5 cm in size, were classified as fibropapillomas by histopathology. All 33 viral-infection-suspected skin samples from roe deer in South Moravia and South Bohemia were PCR positive. The nucleotide sequences of PCR products were 100% homologous to the Western roe deer papillomavirus 1 isolate CcPV-1. Ticks and deer keds from positive roe deer and ticks from dogs were PCR and DNA sequence positive for the roe deer papillomavirus. Viral DNA was also demonstrated in one blood sample from a roe deer female. Differences in metallothionein, zinc, taurine and electrochemical index among samples from lesions and normal skin of affected roe deer and negative controls from papillomavirus-non-infected animals were non-significant. While we have demonstrated circulation of specific roe deer papillomavirus in the Czech Republic that results in multiple fibropapillomatous skin tumours, many ecological and epidemiological issues of this wildlife disease still remain unanswered.

Keywords

Capreolus capreolus; Lipoptena cervi; Ixodes ricinus; papillomatosis; skin tumours; hunting dog

Authors

KRÁL, J.; BANĎOUCHOVÁ, H.; BRICHTA, J.; KOVACOVA, V.; ONDRÁČEK, K.; OSIČKOVÁ, J.; HRUBÁ, H.; HUTAŘOVÁ, Z.; KOMÍNKOVÁ, M.; CERNEI, N.; KONEČNÁ, M.; TMEJOVÁ, K.; ZÍTKA, O.; ADAM, V.; KÍZEK, R.; SKORIC, M.; TREMLOVÁ, B.; TREML, F.; PIKULA, J.

RIV year

2015

Released

1. 6. 2015

Publisher

University of Veterinary and Pharmaceutical Sciences

ISBN

0001-7213

Periodical

ACTA VETERINARIA BRNO

Year of study

84

Number

2

State

Czech Republic

Pages from

105

Pages to

111

Pages count

7

URL

https://actavet.vfu.cz/84/2/0105/

Full text in the Digital Library

http://hdl.handle.net/11012/63714

BibTex

@article{BUT123336,
  author="Jiří {Král} and Hana {Banďouchová} and Jiří {Brichta} and Veronika {Kovacova} and Karel {Ondráček} and Jitka {Osičková} and Hana {Hrubá} and Zdeňka {Hutařová} and Markéta {Komínková} and Natalia Vladimirovna {Cernei} and Marie {Konečná} and Kateřina {Tmejová} and Ondřej {Zítka} and Vojtěch {Adam} and René {Kízek} and Misa {Skoric} and Bohuslava {Tremlová} and František {Treml} and Jiří {Pikula}",
  title="Papillomavirus infection of roe deer in the Czech Republic and fibropapilloma-associated levels of metallothionein, zinc, and oxidative stress",
  journal="ACTA VETERINARIA BRNO",
  year="2015",
  volume="84",
  number="2",
  pages="105--111",
  doi="10.2754/avb201584020105",
  issn="0001-7213",
  url="https://actavet.vfu.cz/84/2/0105/"
}