Publication detail

Novel Oxycellulose/Gelatin Nanofibers for Medical Applications

ŠVACHOVÁ, V. VOJTOVÁ, L. PAVLIŇÁK, D. FOHLEROVÁ, Z.

Original Title

Novel Oxycellulose/Gelatin Nanofibers for Medical Applications

English Title

Novel Oxycellulose/Gelatin Nanofibers for Medical Applications

Type

abstract

Language

en

Original Abstract

Novel hydrolytically stable gelatin nanofibers were modified with sodium or calcium salts of oxidized cellulose (oxycellulose) to enhance nanofibers’ stability and bactericidal efficiency. The unique inhibitory effect of the electrospun gelatin/ oxycellulose nanofibers against pathogenic bacteria was examined by luminometric method using modified bioluminescence of E. coli strain. Non-woven nanofiber fabric has been tested for its biocompatibility using human lung adenocarcinoma cells line NCI-H441. Cells cultured on nanofibrous materials firmly adhered to their surface without the changing in shape, as determined by scanning electron microscopy, and did not show any signs of dying as revealed by live/death staining via fluorescence microscope. This newly developed nanofibrous material seeded with NCI-H441 cell line could be therefore used as a platform for lung disease modeling and anti-cancer drug testing. Moreover, novel bactericidal and hemostatic nanofibers, non-toxic to eukaryotic cells represent a promising option for application in surgery and regenerative medicine, particularly in soft tissue engineering.

English abstract

Novel hydrolytically stable gelatin nanofibers were modified with sodium or calcium salts of oxidized cellulose (oxycellulose) to enhance nanofibers’ stability and bactericidal efficiency. The unique inhibitory effect of the electrospun gelatin/ oxycellulose nanofibers against pathogenic bacteria was examined by luminometric method using modified bioluminescence of E. coli strain. Non-woven nanofiber fabric has been tested for its biocompatibility using human lung adenocarcinoma cells line NCI-H441. Cells cultured on nanofibrous materials firmly adhered to their surface without the changing in shape, as determined by scanning electron microscopy, and did not show any signs of dying as revealed by live/death staining via fluorescence microscope. This newly developed nanofibrous material seeded with NCI-H441 cell line could be therefore used as a platform for lung disease modeling and anti-cancer drug testing. Moreover, novel bactericidal and hemostatic nanofibers, non-toxic to eukaryotic cells represent a promising option for application in surgery and regenerative medicine, particularly in soft tissue engineering.

Keywords

electrospinning, biocompatibility, hydrolytical stability, crosslinking, medicine

Released

03.12.2015

Publisher

Vysoké učení technické v Brně

Location

Brno

ISBN

978-80-214-5290-6

Book

Sborník příspěvků; Studentská konference Chemie je život

Edition

první

Edition number

první

Pages from

367

Pages to

367

Pages count

1

URL

BibTex


@misc{BUT119376,
  author="Veronika {Pavliňáková} and Lucy {Vojtová} and David {Pavliňák} and Zdenka {Fohlerová}",
  title="Novel Oxycellulose/Gelatin Nanofibers for Medical Applications",
  annote="Novel hydrolytically stable gelatin nanofibers were modified with sodium or calcium
salts of oxidized cellulose (oxycellulose) to enhance nanofibers’ stability and bactericidal
efficiency. The unique inhibitory effect of the electrospun gelatin/ oxycellulose nanofibers
against pathogenic bacteria was examined by luminometric method using modified
bioluminescence of E. coli strain. Non-woven nanofiber fabric has been tested for its biocompatibility
using human lung adenocarcinoma cells line NCI-H441. Cells cultured on
nanofibrous materials firmly adhered to their surface without the changing in shape, as determined
by scanning electron microscopy, and did not show any signs of dying as revealed
by live/death staining via fluorescence microscope. This newly developed nanofibrous material
seeded with NCI-H441 cell line could be therefore used as a platform for lung disease
modeling and anti-cancer drug testing. Moreover, novel bactericidal and hemostatic nanofibers,
non-toxic to eukaryotic cells represent a promising option for application in surgery
and regenerative medicine, particularly in soft tissue engineering.",
  address="Vysoké učení technické v Brně",
  booktitle="Sborník příspěvků; Studentská konference Chemie je život",
  chapter="119376",
  edition="první",
  howpublished="online",
  institution="Vysoké učení technické v Brně",
  year="2015",
  month="december",
  pages="367--367",
  publisher="Vysoké učení technické v Brně",
  type="abstract"
}