Publication detail

Dynamic phase differences based on quantitative phase imaging for the objective evaluation of cell behavior

KŘÍŽOVÁ, A. ČOLLÁKOVÁ, J. DOSTÁL, Z. KVASNICA, L. UHLÍŘOVÁ, H. ZIKMUND, T. VESELÝ, P. CHMELÍK, R.

Original Title

Dynamic phase differences based on quantitative phase imaging for the objective evaluation of cell behavior

English Title

Dynamic phase differences based on quantitative phase imaging for the objective evaluation of cell behavior

Type

journal article in Web of Science

Language

en

Original Abstract

Quantitative phase imaging (QPI) brought innovation to noninvasive observation of live cell dynamics seen as cell behavior. Unlike the Zernike phase contrast or differential interference contrast, QPI provides quantitative information about cell dry mass distribution. We used such data for objective evaluation of live cell behavioral dynamics by the advanced method of dynamic phase differences (DPDs). The DPDs method is considered a rational instrument offered by QPI. By subtracting the antecedent from the subsequent image in a time-lapse series, only the changes in mass distribution in the cell are detected. The result is either visualized as a two-dimensional color-coded projection of these two states of the cell or as a time dependence of changes quantified in picograms. Then in a series of time-lapse recordings, the chain of cell mass distribution changes that would otherwise escape attention is revealed. Consequently, new salient features of live cell behavior should emerge. Construction of the DPDs method and results exhibiting the approach are presented. Advantage of the DPDs application is demonstrated on cells exposed to an osmotic challenge. For time-lapse acquisition of quantitative phase images, the recently developed coherence-controlled holographic microscope was employed

English abstract

Quantitative phase imaging (QPI) brought innovation to noninvasive observation of live cell dynamics seen as cell behavior. Unlike the Zernike phase contrast or differential interference contrast, QPI provides quantitative information about cell dry mass distribution. We used such data for objective evaluation of live cell behavioral dynamics by the advanced method of dynamic phase differences (DPDs). The DPDs method is considered a rational instrument offered by QPI. By subtracting the antecedent from the subsequent image in a time-lapse series, only the changes in mass distribution in the cell are detected. The result is either visualized as a two-dimensional color-coded projection of these two states of the cell or as a time dependence of changes quantified in picograms. Then in a series of time-lapse recordings, the chain of cell mass distribution changes that would otherwise escape attention is revealed. Consequently, new salient features of live cell behavior should emerge. Construction of the DPDs method and results exhibiting the approach are presented. Advantage of the DPDs application is demonstrated on cells exposed to an osmotic challenge. For time-lapse acquisition of quantitative phase images, the recently developed coherence-controlled holographic microscope was employed

Keywords

dynamic phase differences; holographic microscopy; live cell behavior evaluation; osmotic challenge; quantitative phase imaging

RIV year

2015

Released

01.11.2015

ISBN

1083-3668

Periodical

JOURNAL OF BIOMEDICAL OPTICS

Year of study

20

Number

11

State

US

Pages from

111214-1

Pages to

111214-8

Pages count

8

Documents

BibTex


@article{BUT116811,
  author="Aneta {Křížová} and Jana {Čolláková} and Zbyněk {Dostál} and Lukáš {Kvasnica} and Hana {Uhlířová} and Tomáš {Zikmund} and Pavel {Veselý} and Radim {Chmelík}",
  title="Dynamic phase differences based on quantitative phase imaging for the objective evaluation of cell behavior",
  annote="Quantitative phase imaging (QPI) brought innovation to noninvasive observation of live cell dynamics seen as cell behavior. Unlike the Zernike phase contrast or differential interference contrast, QPI provides quantitative information about cell dry mass distribution. We used such data for objective evaluation of live cell behavioral dynamics by the advanced method of dynamic phase differences (DPDs). The DPDs method is considered a rational instrument offered by QPI. By subtracting the antecedent from the subsequent image in a time-lapse series, only the changes in mass distribution in the cell are detected. The result is either visualized as a two-dimensional color-coded projection of these two states of the cell or as a time dependence of changes quantified in picograms. Then in a series of time-lapse recordings, the chain of cell mass distribution changes that would otherwise escape attention is revealed. Consequently, new salient features of live cell behavior should emerge. Construction of the DPDs method and results exhibiting the approach are presented. Advantage of the DPDs application is demonstrated on cells exposed to an osmotic challenge. For time-lapse acquisition of quantitative phase images, the recently developed coherence-controlled holographic microscope was employed",
  chapter="116811",
  doi="10.1117/1.JBO.20.11.111214",
  howpublished="online",
  number="11",
  volume="20",
  year="2015",
  month="november",
  pages="111214-1--111214-8",
  type="journal article in Web of Science"
}