Publication detail

Quantitative phase imaging through scattering media by means of coherence-controlled holographic microscope

KOLLÁROVÁ, V. ČOLLÁKOVÁ, J. DOSTÁL, Z. VESELÝ, P. CHMELÍK, R.

Original Title

Quantitative phase imaging through scattering media by means of coherence-controlled holographic microscope

English Title

Quantitative phase imaging through scattering media by means of coherence-controlled holographic microscope

Type

journal article in Web of Science

Language

en

Original Abstract

A coherence-controlled holographic microscope (CCHM) enables quantitative phase imaging with coherent as well as incoherent illumination. The low spatially coherent light induces a coherence gating effect, which makes observation of samples possible also through scattering media. The paper describes theoretically and simulates numerically imaging of a two-dimensional object through a static scattering layer by means of CCHM, with the main focus on the quantitative phase imaging quality. The authors have investigated both strongly and weakly scattering media characterized by different amounts of ballistic and diffuse light. It is demonstrated that the phase information can be revealed also for the case of the static, strongly scattering layer. The dependence of the quality of imaging process on the spatial light coherence is demonstrated. The theoretical calculations and numerical simulations are supported by experimental data gained with a model phase object, as well as living carcinoma cells treated in an optically turbid emulsion

English abstract

A coherence-controlled holographic microscope (CCHM) enables quantitative phase imaging with coherent as well as incoherent illumination. The low spatially coherent light induces a coherence gating effect, which makes observation of samples possible also through scattering media. The paper describes theoretically and simulates numerically imaging of a two-dimensional object through a static scattering layer by means of CCHM, with the main focus on the quantitative phase imaging quality. The authors have investigated both strongly and weakly scattering media characterized by different amounts of ballistic and diffuse light. It is demonstrated that the phase information can be revealed also for the case of the static, strongly scattering layer. The dependence of the quality of imaging process on the spatial light coherence is demonstrated. The theoretical calculations and numerical simulations are supported by experimental data gained with a model phase object, as well as living carcinoma cells treated in an optically turbid emulsion

Keywords

quantitative phase imaging; digital holography; microscopy; coherence-controlled holographic microscopy; imaging through scattering media; imaging through turbid media.

RIV year

2015

Released

01.11.2015

Publisher

SPIE

ISBN

1083-3668

Periodical

JOURNAL OF BIOMEDICAL OPTICS

Year of study

20

Number

11

State

US

Pages from

1112016-1

Pages to

111206-8

Pages count

8

URL

Full text in the Digital Library

Documents

BibTex


@article{BUT115899,
  author="Věra {Kollárová} and Jana {Čolláková} and Zbyněk {Dostál} and Pavel {Veselý} and Radim {Chmelík}",
  title="Quantitative phase imaging through scattering media by means of coherence-controlled holographic microscope",
  annote="A coherence-controlled holographic microscope (CCHM) enables quantitative phase imaging with
coherent as well as incoherent illumination. The low spatially coherent light induces a coherence gating effect,
which makes observation of samples possible also through scattering media. The paper describes theoretically
and simulates numerically imaging of a two-dimensional object through a static scattering layer by means of
CCHM, with the main focus on the quantitative phase imaging quality. The authors have investigated both
strongly and weakly scattering media characterized by different amounts of ballistic and diffuse light. It is
demonstrated that the phase information can be revealed also for the case of the static, strongly scattering
layer. The dependence of the quality of imaging process on the spatial light coherence is demonstrated.
The theoretical calculations and numerical simulations are supported by experimental data gained with a model
phase object, as well as living carcinoma cells treated in an optically turbid emulsion",
  address="SPIE",
  chapter="115899",
  doi="10.1117/1.JBO.20.11.111206",
  howpublished="print",
  institution="SPIE",
  number="11",
  volume="20",
  year="2015",
  month="november",
  pages="1112016-1--111206-8",
  publisher="SPIE",
  type="journal article in Web of Science"
}