Publication detail

The comparison of chemical and magnetic channelrhodopsin-2 transfection efficiency

BAIAZITOVA, L. SVOBODA, O. ČMIEL, V. PROVAZNÍK, I. FOHLEROVÁ, Z. HUBÁLEK, J.

Original Title

The comparison of chemical and magnetic channelrhodopsin-2 transfection efficiency

English Title

The comparison of chemical and magnetic channelrhodopsin-2 transfection efficiency

Type

conference paper

Language

en

Original Abstract

In this work the chemical (polyethylenimine) and magnetic nanoparticle (MATRA) based transfection methods of channelrhodopsin-2 to HEK293 cell has been compared. We optimized transfection protocol by changing reagent and DNA amounts and the most appropriate fluorescent results were obtained after 24 hours of incubation from transfection time.

English abstract

In this work the chemical (polyethylenimine) and magnetic nanoparticle (MATRA) based transfection methods of channelrhodopsin-2 to HEK293 cell has been compared. We optimized transfection protocol by changing reagent and DNA amounts and the most appropriate fluorescent results were obtained after 24 hours of incubation from transfection time.

Keywords

HEK293 cells, transfection, channelrhodopsin-2, polyethylenimine, MATRA-A reagent

RIV year

2015

Released

26.05.2015

Publisher

Masarykova Univerzita - MUNI PRESS

Location

Brno, Czech Republic

ISBN

978-80-210-7857-4

Book

XV. Workshop of Physical Chemists and Electrochemists

Edition

1

Edition number

1

Pages from

71

Pages to

73

Pages count

3

BibTex


@inproceedings{BUT115179,
  author="Larisa {Baiazitova} and Ondřej {Svoboda} and Vratislav {Čmiel} and Ivo {Provazník} and Zdenka {Fohlerová} and Jaromír {Hubálek}",
  title="The comparison of chemical and magnetic channelrhodopsin-2 transfection efficiency",
  annote="In this work the chemical (polyethylenimine) and magnetic nanoparticle (MATRA) based transfection methods of channelrhodopsin-2 to HEK293 cell has been compared. We optimized transfection protocol by changing reagent and DNA amounts and the most appropriate fluorescent results were obtained after 24 hours of incubation from transfection time.",
  address="Masarykova Univerzita - MUNI PRESS",
  booktitle="XV. Workshop of Physical Chemists and Electrochemists",
  chapter="115179",
  edition="1",
  howpublished="electronic, physical medium",
  institution="Masarykova Univerzita - MUNI PRESS",
  year="2015",
  month="may",
  pages="71--73",
  publisher="Masarykova Univerzita - MUNI PRESS",
  type="conference paper"
}