Publication detail

Interaction study of arsenic (III and V) ions with metallothioneingene (MT2A) fragment

NEJDL, L. SKALIČKOVÁ, S. KUDR, J. RUTTKAY-NEDECKÝ, B. DOSTÁLOVÁ, S. KREMPLOVÁ, M. KENŠOVÁ, R. MOULICK, A. NOVOTNÁ, M. ADAM, V. KIZEK, R.

Original Title

Interaction study of arsenic (III and V) ions with metallothioneingene (MT2A) fragment

Type

journal article in Web of Science

Language

English

Original Abstract

Arsenic compounds belong to the most controversial agents concerning human health. Arsenic (As) isconsidered as a top environmental element influencing human health due to its adverse effects includ-ing cancer, diabetes, cardiovascular disease, and reproductive or developmental problems. Despite theproven mutagenic, teratogenic and carcinogenic effects, the arsenic compounds are used for centuriesto treat infectious diseases. In our work, we focused on studying of interactions of As(III) and/or As(V)with DNA. Interactions between arsenic ions and DNA were monitored by UV/vis spectrophotometryby measuring absorption and fluorescence spectra, atomic absorption spectrometry, electrochemicalmeasurements (square wave voltammetry) and agarose gel electrophoresis. Using these methods, weobserved a stable structure of DNA with As(III) within the concentration range 0.4–6.25 g mL - 1. HigherAs(III) concentration caused degradation of DNA. However, similar effects were not observed for As(V).

Keywords

Arsenic, Spectrometry, Electrochemistry, DNA Interaction, Anticancer drug

Authors

NEJDL, L.; SKALIČKOVÁ, S.; KUDR, J.; RUTTKAY-NEDECKÝ, B.; DOSTÁLOVÁ, S.; KREMPLOVÁ, M.; KENŠOVÁ, R.; MOULICK, A.; NOVOTNÁ, M.; ADAM, V.; KIZEK, R.

RIV year

2015

Released

1. 1. 2015

ISBN

0141-8130

Periodical

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Year of study

72

Number

1

State

Kingdom of the Netherlands

Pages from

599

Pages to

605

Pages count

7

BibTex

@article{BUT112400,
  author="Lukáš {Nejdl} and Sylvie {Skaličková} and Jiří {Kudr} and Branislav {Ruttkay-Nedecký} and Simona {Dostálová} and Monika {Kremplová} and Renáta {Kenšová} and Amitava {Moulick} and Marie {Novotná} and Vojtěch {Adam} and René {Kizek}",
  title="Interaction study of arsenic (III and V) ions with metallothioneingene (MT2A) fragment",
  journal="INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES",
  year="2015",
  volume="72",
  number="1",
  pages="599--605",
  doi="10.1016/j.ijbiomac.2014.09.001",
  issn="0141-8130"
}