Publication detail

Effect of Ampicillin, Streptomycin, Penicillin and Tetracycline on Metal Resistant and Non-Resistant Staphylococcus aureus

CHUDOBOVÁ, D. DOSTÁLOVÁ, S. BLAŽKOVÁ, I. MICHÁLEK, P. RUTTKAY-NEDECKÝ, B. SKLENÁŘ, M. NEJDL, L. KUDR, J. GUMULEC, J. TMEJOVÁ, K. KONEČNÁ, M. VACULOVIČOVÁ, M. HYNEK, D. MASAŘÍK, M. KYNICKÝ, J. KIZEK, R. ADAM, V.

Original Title

Effect of Ampicillin, Streptomycin, Penicillin and Tetracycline on Metal Resistant and Non-Resistant Staphylococcus aureus

Type

journal article in Web of Science

Language

English

Original Abstract

There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections. The aim of this study was to compare the effects of antibiotics on Staphylococcus aureus non-resistant strain and strains resistant to cadmium or lead ions. Metal resistant strains were created by the gradual addition of 2 mM solution of metal ions (cadmium or lead) to the S. aureus culture. An increasing antimicrobial effect of ampicillin, streptomycin, penicillin and tetracycline (0, 10, 25, 50, 75, 150, 225 and 300 mu M) on the resistant strains was observed using a method of growth curves. A significant growth inhibition (compared to control) of cadmium resistant cells was observed in the presence of all the four different antibiotics. On the other hand, the addition of streptomycin and ampicillin did not inhibit the growth of lead resistant strain. Other antibiotics were still toxic to the bacterial cells. Significant differences in the morphology of cell walls were indicated by changes in the cell shape. Our data show that the presence of metal ions in the urban environment may contribute to the development of bacterial strain resistance to other substances including antibiotics, which would have an impact on public health.

Keywords

S. aureus; antimicrobial resistance; antibiotics; metal resistance; cross resistance; growth curves; inhibition concentrations; spectrophotometry

Key words in English

S. aureus; antimicrobial resistance; antibiotics; metal resistance; cross resistance; growth curves; inhibition concentrations; spectrophotometry

Authors

CHUDOBOVÁ, D.; DOSTÁLOVÁ, S.; BLAŽKOVÁ, I.; MICHÁLEK, P.; RUTTKAY-NEDECKÝ, B.; SKLENÁŘ, M.; NEJDL, L.; KUDR, J.; GUMULEC, J.; TMEJOVÁ, K.; KONEČNÁ, M.; VACULOVIČOVÁ, M.; HYNEK, D.; MASAŘÍK, M.; KYNICKÝ, J.; KIZEK, R.; ADAM, V.

RIV year

2014

Released

19. 3. 2014

Publisher

MDPI AG, POSTFACH

Location

CH-4005 BASEL, SWITZERLAND

ISBN

1660-4601

Periodical

International Journal of Environmental Research and Public Health

Year of study

2014

Number

3

State

Swiss Confederation

Pages from

3233

Pages to

3255

Pages count

23

URL

http://www.mdpi.com/1660-4601/11/3/3233

Full text in the Digital Library

http://hdl.handle.net/11012/63711

BibTex

@article{BUT112144,
  author="Dagmar {Hegerová} and Simona {Dostálová} and Iva {Blažková} and Petr {Michálek} and Branislav {Ruttkay-Nedecký} and Matěj {Sklenář} and Lukáš {Nejdl} and Jiří {Kudr} and Jaromír {Gumulec} and Kateřina {Tmejová} and Marie {Konečná} and Markéta {Vaculovičová} and David {Hynek} and Michal {Masařík} and Jindřich {Kynický} and René {Kizek} and Vojtěch {Adam}",
  title="Effect of Ampicillin, Streptomycin, Penicillin and Tetracycline on Metal Resistant and Non-Resistant Staphylococcus aureus",
  journal="International Journal of Environmental Research and Public Health",
  year="2014",
  volume="2014",
  number="3",
  pages="3233--3255",
  doi="10.3390/ijerph110303233",
  issn="1660-4601",
  url="http://www.mdpi.com/1660-4601/11/3/3233"
}