Publication detail

gamma-Fe2O3 Magnetic Core Functionalized with Tetraethyl Orthosilicate and 3-Aminopropyl Triethoxysilane for an Isolation of H7N7 Influenza Serotype Virions

HEGER, Z. CERNEI, N. GURÁŇ, R. MICHÁLEK, P. MILOSAVLJEVIĆ, V. KOPEL, P. ZÍTKA, O. KYNICKÝ, J. LÁNY, P. ADAM, V. KIZEK, R.

Original Title

gamma-Fe2O3 Magnetic Core Functionalized with Tetraethyl Orthosilicate and 3-Aminopropyl Triethoxysilane for an Isolation of H7N7 Influenza Serotype Virions

Type

journal article in Web of Science

Language

English

Original Abstract

The influenza viruses cause annual epidemics of respiratory disease and occasional pandemics, which constitute a major public-health issue. The most important tool in the diagnosis of influenza pandemic is timely and right diagnosis. Hence, this study presents synthesis of paramagnetic particles formed by nanomaghemite (gamma-Fe2O3) core, whose surface was functionalized with tetraethyl orthosilicate (TEOS) and 3-aminopropyl triethoxysilane (APTES), able to bind H7N7 influenza virions. Paramagnetic particles show the ability to immobilize virions for direct analysis using ion-exchange chromatography with VIS detection. The presence of H7N7 virions on surface of paramagnetic beads was confirmed by matrix-assisted laser desorption/ionization time of flight mass spectrometry, based upon the mass weight of H7N7 virions, determined before and after establishment of binding. Moreover, using scanning electrochemical microscopy revealed, that binding is provided due to strong charge-charge interaction between positively charged influenza surface protein hemagglutinin and negatively charged surface of nanocomposite. It can be concluded that paramagnetic beads show significant binding capability towards H7N7 virions.

Keywords

H7N7; Functionalization; Lab-on-a-Chip; Magnetic immobilization; Sensor

Authors

HEGER, Z.; CERNEI, N.; GURÁŇ, R.; MICHÁLEK, P.; MILOSAVLJEVIĆ, V.; KOPEL, P.; ZÍTKA, O.; KYNICKÝ, J.; LÁNY, P.; ADAM, V.; KIZEK, R.

RIV year

2014

Released

1. 7. 2014

ISBN

1452-3981

Periodical

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Year of study

9

Number

7

State

Republic of Serbia

Pages from

3374

Pages to

3385

Pages count

12

BibTex

@article{BUT111176,
  author="Zbyněk {Heger} and Natalia Vladimirovna {Cernei} and Roman {Guráň} and Petr {Michálek} and Vedran {Milosavljević} and Pavel {Kopel} and Ondřej {Zítka} and Jindřich {Kynický} and Petr {Lány} and Vojtěch {Adam} and René {Kizek}",
  title="gamma-Fe2O3 Magnetic Core Functionalized with Tetraethyl Orthosilicate and 3-Aminopropyl Triethoxysilane for an Isolation of H7N7 Influenza Serotype Virions",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2014",
  volume="9",
  number="7",
  pages="3374--3385",
  issn="1452-3981"
}