Publication detail

Time-Resolved Fluorescence Spectra of Different Soil Humic Acids and Standard IHSS Elliott Soil

ENEV, V. KLUČÁKOVÁ, M. DOSKOČIL, L.

Original Title

Time-Resolved Fluorescence Spectra of Different Soil Humic Acids and Standard IHSS Elliott Soil

English Title

Time-Resolved Fluorescence Spectra of Different Soil Humic Acids and Standard IHSS Elliott Soil

Type

abstract

Language

en

Original Abstract

The aim of this work was study structure and dynamics of different soil humic acids (HAs). Object of our study were three samples HAs which were isolated from sandy soil – Arenosols (locality Ratíškovice, Czech Republic), Chernozem – Haplic Luvisol (locality Praha-Ruzyně, Crop Research Institute, Czech Republic) and standard HA Elliott Soil (1S102H). Isolation of soil HAs were performed according to the procedure recommended by the International Humic Substances Society (IHSS). All samples of soil HAs were characterized by Steady-State and Time-Resolved Fluorescence Spectroscopy. Emission wavelength dependent fluorescence decays are used to construct Time-Resolved Fluorescence Spectra (TRES) of soil humic acids, which are useful to obtained information on the excited state kinetics and heterogeneity of emissive species in a system. Time resolved fluorescence spectra (TRES), plotted as fluorescence intensity vs. wavelength, were constructed using alfai (wavelength) and taui (wavelength), and steady-state emission spectrum corrected for the quantum efficiency of the photomultiplier. Fluorescence spectra were recorded in aqueous solutions of 10 mg/L HAs after overnight equilibration at room temperature, using FluoroLog luminescence spectrophotometer. The pH-value of the samples was adjusted to seven using a standard phosphate buffer. Steady-State emission spectra were recorded over the range of 390–600 nm at a constant excitation wavelength of 329 nm. Time-resolved fluorescence measurements were carried out by time-correlated single-photon counting (TCSPC) method. Fluorescence decay at each wavelength was deconvoluted using the instrument response function and a multiexponential function (three exponential functions). The fluorescence intensity values (in counts per second – CPS) of samples were corrected using method of Lakowicz.

English abstract

The aim of this work was study structure and dynamics of different soil humic acids (HAs). Object of our study were three samples HAs which were isolated from sandy soil – Arenosols (locality Ratíškovice, Czech Republic), Chernozem – Haplic Luvisol (locality Praha-Ruzyně, Crop Research Institute, Czech Republic) and standard HA Elliott Soil (1S102H). Isolation of soil HAs were performed according to the procedure recommended by the International Humic Substances Society (IHSS). All samples of soil HAs were characterized by Steady-State and Time-Resolved Fluorescence Spectroscopy. Emission wavelength dependent fluorescence decays are used to construct Time-Resolved Fluorescence Spectra (TRES) of soil humic acids, which are useful to obtained information on the excited state kinetics and heterogeneity of emissive species in a system. Time resolved fluorescence spectra (TRES), plotted as fluorescence intensity vs. wavelength, were constructed using alfai (wavelength) and taui (wavelength), and steady-state emission spectrum corrected for the quantum efficiency of the photomultiplier. Fluorescence spectra were recorded in aqueous solutions of 10 mg/L HAs after overnight equilibration at room temperature, using FluoroLog luminescence spectrophotometer. The pH-value of the samples was adjusted to seven using a standard phosphate buffer. Steady-State emission spectra were recorded over the range of 390–600 nm at a constant excitation wavelength of 329 nm. Time-resolved fluorescence measurements were carried out by time-correlated single-photon counting (TCSPC) method. Fluorescence decay at each wavelength was deconvoluted using the instrument response function and a multiexponential function (three exponential functions). The fluorescence intensity values (in counts per second – CPS) of samples were corrected using method of Lakowicz.

Keywords

Soil Humic Acids, Time-Resolved Fluorescence Spectroscopy, Steady-State Fluorescence Spectroscopy, Time-Resolved Emission Spectra (TRES), Bathochromic Shift of Fluorescence Maximum

Released

05.11.2014

Publisher

TANGER Ltd., Keltičkova 62, 710 00, Ostrava, Česká Republika, EU

Location

AMOS repro Ltd., Čs. Legií 8, 702 00 Ostrava, Česká republika, EU

ISBN

978-80-87294-55-0

Book

Conference Proceedings, 6th International Conference November 5th–7th 2014, Hotel Voronez 1, Brno, Czech Republic, EU

Edition

2014 TANGER Ltd., Ostrava

Edition number

1st Edition, 2014

Pages from

168

Pages to

169

Pages count

2

BibTex


@misc{BUT110283,
  author="Vojtěch {Enev} and Martina {Klučáková} and Leoš {Doskočil}",
  title="Time-Resolved Fluorescence Spectra of Different Soil Humic Acids and Standard IHSS Elliott Soil",
  annote="The aim of this work was study structure and dynamics of different soil humic acids (HAs). Object of our study were three samples HAs which were isolated from sandy soil – Arenosols (locality Ratíškovice, Czech Republic), Chernozem – Haplic Luvisol (locality Praha-Ruzyně, Crop Research Institute, Czech Republic) and standard HA Elliott Soil (1S102H). Isolation of soil HAs were performed according to the procedure recommended by the International Humic Substances Society (IHSS). All samples of soil HAs were characterized by Steady-State and Time-Resolved Fluorescence Spectroscopy. Emission wavelength dependent fluorescence decays are used to construct Time-Resolved Fluorescence Spectra (TRES) of soil humic acids, which are useful to obtained information on the excited state kinetics and heterogeneity of emissive species in a system. Time resolved fluorescence spectra (TRES), plotted as fluorescence intensity vs. wavelength, were constructed using alfai (wavelength) and taui (wavelength), and steady-state emission spectrum corrected for the quantum efficiency of the photomultiplier. Fluorescence spectra were recorded in aqueous solutions of 10 mg/L HAs after overnight equilibration at room temperature, using FluoroLog luminescence spectrophotometer. The pH-value of the samples was adjusted to seven using a standard phosphate buffer. Steady-State emission spectra were recorded over the range of 390–600 nm at a constant excitation wavelength of 329 nm. Time-resolved fluorescence measurements were carried out by time-correlated single-photon counting (TCSPC) method. Fluorescence decay at each wavelength was deconvoluted using the instrument response function and a multiexponential function (three exponential functions). The fluorescence intensity values (in counts per second – CPS) of samples were corrected using method of Lakowicz.",
  address="TANGER Ltd., Keltičkova 62, 710 00, Ostrava, Česká Republika, EU",
  booktitle="Conference Proceedings, 6th International Conference November 5th–7th 2014, Hotel Voronez 1, Brno, Czech Republic, EU",
  chapter="110283",
  edition="2014 TANGER Ltd., Ostrava",
  howpublished="print",
  institution="TANGER Ltd., Keltičkova 62, 710 00, Ostrava, Česká Republika, EU",
  year="2014",
  month="november",
  pages="168--169",
  publisher="TANGER Ltd., Keltičkova 62, 710 00, Ostrava, Česká Republika, EU",
  type="abstract"
}