Publication detail

Effect of CTAB and CTAB in the presence of hyaluronan on selected human cell types

KALBÁČOVÁ, M. VERDÁNOVÁ, M. MRAVEC, F. HALASOVÁ, T. PEKAŘ, M.

Original Title

Effect of CTAB and CTAB in the presence of hyaluronan on selected human cell types

English Title

Effect of CTAB and CTAB in the presence of hyaluronan on selected human cell types

Type

journal article in Web of Science

Language

en

Original Abstract

The effect of different concentrations of CTAB (in the range of 0.2 mM – 2 mM, i.e. including its critical micelle concentration) on viability of selected human cells (osteoblasts and keratinocytes) was studied by a variety of methods (immunocytochemical and biochemical), testing the cell viability and the metabolism, to get a complex overview. All of the used methods confirmed the cytotoxic effect of CTAB, which could, however, be suppressed by the presence of hyaluronan (molecular weight 806 kDa, in the concentration of 1 g/l) in the case of the lowest CTAB concentration used (0.2 mM) when the fetal bovine serum was also present in the cultivation medium. Thus it could be concluded that hyaluronan can be used as successful protector of specific cell types against cytotoxic CTAB at low concentrations.

English abstract

The effect of different concentrations of CTAB (in the range of 0.2 mM – 2 mM, i.e. including its critical micelle concentration) on viability of selected human cells (osteoblasts and keratinocytes) was studied by a variety of methods (immunocytochemical and biochemical), testing the cell viability and the metabolism, to get a complex overview. All of the used methods confirmed the cytotoxic effect of CTAB, which could, however, be suppressed by the presence of hyaluronan (molecular weight 806 kDa, in the concentration of 1 g/l) in the case of the lowest CTAB concentration used (0.2 mM) when the fetal bovine serum was also present in the cultivation medium. Thus it could be concluded that hyaluronan can be used as successful protector of specific cell types against cytotoxic CTAB at low concentrations.

Keywords

Cetyltrimethylammonium bromide; Cytoprotection; Cytotoxicity; Hyaluronan; Keratinocyte; Osteoblast

RIV year

2014

Released

08.09.2014

Pages from

204

Pages to

208

Pages count

5

BibTex


@article{BUT109678,
  author="Filip {Mravec} and Tereza {Venerová} and Miloslav {Pekař}",
  title="Effect of CTAB and CTAB in the presence of hyaluronan on selected human cell types",
  annote="The effect of different concentrations of CTAB (in the range of 0.2 mM – 2 mM, i.e. including its critical micelle concentration) on viability of selected human cells (osteoblasts and keratinocytes) was studied by a variety of methods (immunocytochemical and biochemical), testing the cell viability and the metabolism, to get a complex overview. All of the used methods confirmed the cytotoxic effect of CTAB, which could, however, be suppressed by the presence of hyaluronan (molecular weight 806 kDa, in the concentration of 1 g/l) in the case of the lowest CTAB concentration used (0.2 mM) when the fetal bovine serum was also present in the cultivation medium. Thus it could be concluded that hyaluronan can be used as successful protector of specific cell types against cytotoxic CTAB at low concentrations.",
  chapter="109678",
  number="1",
  volume="460",
  year="2014",
  month="september",
  pages="204--208",
  type="journal article in Web of Science"
}