Publication detail

Spectral Characterization of Selected Natural Humic Substances

ENEV, V. POSPÍŠILOVÁ, Ľ. KLUČÁKOVÁ, M. LIPTAJ, T. DOSKOČIL, L.

Original Title

Spectral Characterization of Selected Natural Humic Substances

English Title

Spectral Characterization of Selected Natural Humic Substances

Type

journal article

Language

en

Original Abstract

Current concern for soil quality has stimulated research on soil organic matter (OM). Humic substances (HS) of different origin were compared applying UV/VIS spectroscopy, Fourier transform infrared spectroscopy (FTIR), fluorescence spectroscopy, and 13C nuclear magnetic resonance (13C NMR). Sodium humates samples were isolated from soil (Gleyic Luvisol), compost, and South Moravian lignite from the mine Mír in Mikulčice. Sodium humates (SH) were extracted by a conventional procedure recommended by the International Humic Substances Society (IHSS). Results showed that the presence of O containing functional groups (carbonyl in aldehydes and ketones, carboxyl in carboxylic acids, ester and ether groups) are in the order of compost > soil > lignohumate > lignite. Further, results of FTIR, fluorescence spectroscopy, and 13C NMR suggested that samples of sodium humates isolated from soil, compost, and lignite were a more polycondensed, oxidized, unsaturated, humified, and aromatic structure. On the other hand, commercial lignohumate (LH) had very simple structural components and wide molecular heterogeneity. Furthermore, a small molecular size and weight, low degree of aromatic polycondensation, low level of conjugated chromophores and fluorophores, and low humification degree were characteristic for commercial LH. It should be noted that the sample of commercial LH was characterized by 13C NMR analysis with a slightly higher value of aromaticity in comparison with the sample of compost. The application of nondestructive analytical methods such as UV/VIS, FTIR, 13C NMR, and fluorescence spectroscopy help us to provide main characteristics of selected humic substances.

English abstract

Current concern for soil quality has stimulated research on soil organic matter (OM). Humic substances (HS) of different origin were compared applying UV/VIS spectroscopy, Fourier transform infrared spectroscopy (FTIR), fluorescence spectroscopy, and 13C nuclear magnetic resonance (13C NMR). Sodium humates samples were isolated from soil (Gleyic Luvisol), compost, and South Moravian lignite from the mine Mír in Mikulčice. Sodium humates (SH) were extracted by a conventional procedure recommended by the International Humic Substances Society (IHSS). Results showed that the presence of O containing functional groups (carbonyl in aldehydes and ketones, carboxyl in carboxylic acids, ester and ether groups) are in the order of compost > soil > lignohumate > lignite. Further, results of FTIR, fluorescence spectroscopy, and 13C NMR suggested that samples of sodium humates isolated from soil, compost, and lignite were a more polycondensed, oxidized, unsaturated, humified, and aromatic structure. On the other hand, commercial lignohumate (LH) had very simple structural components and wide molecular heterogeneity. Furthermore, a small molecular size and weight, low degree of aromatic polycondensation, low level of conjugated chromophores and fluorophores, and low humification degree were characteristic for commercial LH. It should be noted that the sample of commercial LH was characterized by 13C NMR analysis with a slightly higher value of aromaticity in comparison with the sample of compost. The application of nondestructive analytical methods such as UV/VIS, FTIR, 13C NMR, and fluorescence spectroscopy help us to provide main characteristics of selected humic substances.

Keywords

13C NMR spectroscopy, Fourier transform infrared spectroscopy (FTIR), humates, lignohumate, steady-state fluorescence spectroscopy, UV-VIS

RIV year

2014

Released

27.01.2014

Publisher

Czech Academy of Agricultural Sciences

Location

Prague

Pages from

9

Pages to

17

Pages count

9

BibTex


@article{BUT105185,
  author="Vojtěch {Enev} and Martina {Klučáková} and Leoš {Doskočil}",
  title="Spectral Characterization of Selected Natural Humic Substances",
  annote="Current concern for soil quality has stimulated research on soil organic matter (OM). Humic substances (HS)
of different origin were compared applying UV/VIS spectroscopy, Fourier transform infrared spectroscopy
(FTIR), fluorescence spectroscopy, and 13C nuclear magnetic resonance (13C NMR).
Sodium humates samples were isolated from soil (Gleyic Luvisol), compost, and South Moravian lignite from
the mine Mír in Mikulčice. Sodium humates (SH) were extracted by a conventional procedure recommended by
the International Humic Substances Society (IHSS). Results showed that the presence of O containing functional
groups (carbonyl in aldehydes and ketones, carboxyl in carboxylic acids, ester and ether groups) are in the order
of compost > soil > lignohumate > lignite. Further, results of FTIR, fluorescence spectroscopy, and 13C NMR
suggested that samples of sodium humates isolated from soil, compost, and lignite were a more polycondensed,
oxidized, unsaturated, humified, and aromatic structure. On the other hand, commercial lignohumate (LH) had
very simple structural components and wide molecular heterogeneity. Furthermore, a small molecular size and
weight, low degree of aromatic polycondensation, low level of conjugated chromophores and fluorophores, and
low humification degree were characteristic for commercial LH. It should be noted that the sample of commercial
LH was characterized by 13C NMR analysis with a slightly higher value of aromaticity in comparison with the
sample of compost. The application of nondestructive analytical methods such as UV/VIS, FTIR, 13C NMR,
and fluorescence spectroscopy help us to provide main characteristics of selected humic substances.",
  address="Czech Academy of Agricultural Sciences",
  chapter="105185",
  institution="Czech Academy of Agricultural Sciences",
  number="1",
  volume="9",
  year="2014",
  month="january",
  pages="9--17",
  publisher="Czech Academy of Agricultural Sciences",
  type="journal article"
}